After an overnight fast, animal received a single intraperitoneal dose of corn oil and sacrificed 8 h later.
RNeasy kit, DNase I treatment on column, following manufacturer's instructions.
Biotinylated cRNA were prepared according to the standard Affymetrix protocol
Following fragmentation, cRNA were hybridized at 45c for 16 h using a Affymetrix GeneChip Hybridization Oven 640
Affymetrix GeneChip Scanner 3000
Gene expression data from adult rat liver; corn oil-control; 8 h treatment
Affymetrix Microarray Analysis Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.