|
| Status |
Public on Jun 03, 2006 |
| Title |
corn oil control, biological rep5b |
| Sample type |
RNA |
| |
|
| Source name |
Rattus, adult liver
|
| Organism |
Rattus norvegicus |
| Characteristics |
Sprague-Dawley, 150-200 g
|
| Treatment protocol |
After an overnight fast, animal received a single intraperitoneal dose of corn oil and sacrificed 8 h later
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy kit, DNase I treatment on column, following manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA were hybridized at 45c for 16 h using a Affymetrix GeneChip Hybridization Oven 640
|
| Scan protocol |
Affymetrix Microarray Analysis Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| Description |
Gene expression data from adult rat liver; corn oil-control; 8 h treatment
|
| Data processing |
Affymetrix Microarray Analysis Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
May 31, 2006 |
| Last update date |
Jun 02, 2006 |
| Contact name |
Linda C Quattrochi |
| E-mail(s) |
linda.quattrochi@uchsc.edu
|
| Phone |
303-315-3522
|
| Organization name |
University of Colorado at Denver and Health Sciences
|
| Department |
Medicine
|
| Lab |
Quattrochi
|
| Street address |
4200 E. 9th Ave, B146
|
| City |
Denver |
| State/province |
CO |
| ZIP/Postal code |
80262 |
| Country |
USA |
| |
|
| Platform ID |
GPL342 |
| Series (1) |
| GSE4959 |
Expression data from adult Rattus liver |
|
