|
| Status |
Public on Jun 12, 2013 |
| Title |
Liver_0d |
| Sample type |
RNA |
| |
|
| Source name |
liver, uninfected
|
| Organism |
Mus musculus |
| Characteristics |
background strain: BALB/c
gender: female
tissue: liver
infectious agent: n/a (uninfected)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the RNeasy Mini kit (Qiagen Inc., Valencia, CA, USA) and DNA was removed by on-column DNase digestion with the RNase-Free DNase set (Qiagen). RNA quality and concentration was determined by analysis with an Agilent 2100 bioanalyzer.
|
| Label |
Cy3
|
| Label protocol |
cDNA synthesis followed by Cy3-labeling
|
| |
|
| Hybridization protocol |
Cy3-labeled cDNA was hybridized overnight at 42°C to mus musculus 12x135K Array (Roche NimbleGen)
|
| Scan protocol |
Arrays were scanned with the Axon GenePix 4000B microarray scanner
|
| Data processing |
The raw data (.pair file) was subjected to RMA (Robust Multi-Array Analysis; Irizarry et al. Biostatistics 4(2):249), quantile normalization (Bolstad et al. Bioinformatics 19(2):185), and background correction as implemented in the NimbleScan software package, version 2.5 (Roche NimbleGen, Inc.).
|
| |
|
| Submission date |
Apr 11, 2013 |
| Last update date |
Jun 12, 2013 |
| Contact name |
Pengfei Cai |
| E-mail(s) |
caipengfei@ipbcams.ac.cn
|
| Organization name |
Institute of Pathogen Biology, Chinese Academy of Medical Sciences
|
| Lab |
MOH Key Laboratory of Systems Biology of Pathogens
|
| Street address |
#9 Dong Dan San Tiao
|
| City |
Beijing |
| ZIP/Postal code |
100730 |
| Country |
China |
| |
|
| Platform ID |
GPL10192 |
| Series (1) |
| GSE45985 |
MicroRNA-gene expression network in murine liver during Schistosoma japonicum infection |
|
