|
| Status |
Public on Apr 19, 2013 |
| Title |
GFP4_1578J |
| Sample type |
RNA |
| |
|
| Source name |
adipocytes expressing GFP
|
| Organism |
Mus musculus |
| Characteristics |
cell type: F442A cultured adipocyte
cell line: 3T3L1
back ground strain: Swiss
|
| Treatment protocol |
Mature (day 6- 8 differentiation) adipocytes were infected with adenovirus expressing sXBP1 or GFP. After 4-6 days of infection cells were harvested for analysis.
|
| Growth protocol |
F442A preadipocytes were differentiated according to standard adipogenesis protocol. (see Gregor et al., Cell Reports 2013)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Affymetrix protocol
|
| |
|
| Hybridization protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol. (Affymetrix GeneChip Manual (rev.1.3))
|
| Scan protocol |
The samples were hybridized and scanned according to Affymetrix protocol for Mouse 430A 2.0 chips. (Affymetrix GeneChip Manual (rev.1.3))
|
| Description |
gene expression data from adipocytes overexpressing GFP
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) as normalization method. Genome profiling was performed at Harvard Medical School-Partners Healthcare Center for Genetics and Genomics using an Affymetrics platform (Cambridge, MA).
|
| |
|
| Submission date |
Apr 18, 2013 |
| Last update date |
Apr 19, 2013 |
| Contact name |
Margaret Gregor |
| E-mail(s) |
mgustafs@hsph.harvard.edu
|
| Organization name |
Harvard School of Public Health
|
| Department |
Genetics and Complex Diseases
|
| Lab |
Laboratory of Dr. Gokhan Hotamisligil
|
| Street address |
665 Huntington Ave
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL8321 |
| Series (1) |
| GSE46178 |
Overexpression of spliced XBP1 in cultured Adipocytes |
|
