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Sample GSM1125691 Query DataSets for GSM1125691
Status Public on Apr 19, 2013
Title sXBP2_1578R
Sample type RNA
 
Source name adipocytes expressing spliced XBP1
Organism Mus musculus
Characteristics cell type: F442A cultured adipocyte
cell line: 3T3L1
back ground strain: Swiss
Treatment protocol Mature (day 6- 8 differentiation) adipocytes were infected with adenovirus expressing sXBP1 or GFP. After 4-6 days of infection cells were harvested for analysis.
Growth protocol F442A preadipocytes were differentiated according to standard adipogenesis protocol. (see Gregor et al., Cell Reports 2013)
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Affymetrix protocol
 
Hybridization protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol. (Affymetrix GeneChip Manual (rev.1.3))
Scan protocol The samples were hybridized and scanned according to Affymetrix protocol for Mouse 430A 2.0 chips. (Affymetrix GeneChip Manual (rev.1.3))
Description gene expression data from adipocytes overexpressing spliced XBP1
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) as normalization method. Genome profiling was performed at Harvard Medical School-Partners Healthcare Center for Genetics and Genomics using an Affymetrics platform (Cambridge, MA).
 
Submission date Apr 18, 2013
Last update date Apr 19, 2013
Contact name Margaret Gregor
E-mail(s) mgustafs@hsph.harvard.edu
Organization name Harvard School of Public Health
Department Genetics and Complex Diseases
Lab Laboratory of Dr. Gokhan Hotamisligil
Street address 665 Huntington Ave
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL8321
Series (1)
GSE46178 Overexpression of spliced XBP1 in cultured Adipocytes

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity
ABS_CALL

Data table
ID_REF VALUE ABS_CALL
AFFX-BioB-5_at 182.4 P
AFFX-BioB-M_at 231 P
AFFX-BioB-3_at 185.1 P
AFFX-BioC-5_at 448.5 P
AFFX-BioC-3_at 573.7 P
AFFX-BioDn-5_at 1792.4 P
AFFX-BioDn-3_at 2888.1 P
AFFX-CreX-5_at 8565.5 P
AFFX-CreX-3_at 8974 P
AFFX-DapX-5_at 6.1 A
AFFX-DapX-M_at 1.9 A
AFFX-DapX-3_at 22.3 A
AFFX-LysX-5_at 15.4 A
AFFX-LysX-M_at 8.1 A
AFFX-LysX-3_at 14.3 A
AFFX-PheX-5_at 2 A
AFFX-PheX-M_at 2.6 A
AFFX-PheX-3_at 44.8 P
AFFX-ThrX-5_at 19.4 A
AFFX-ThrX-M_at 14 A

Total number of rows: 22690

Table truncated, full table size 421 Kbytes.




Supplementary file Size Download File type/resource
GSM1125691_sXBP2_1578R.CEL.gz 2.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table
Processed data are available on Series record

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