|
| Status |
Public on Sep 06, 2006 |
| Title |
Neuroblastoma_CL_IMR5 |
| Sample type |
SAGE |
| Anchor |
NlaIII
|
| Tag Count |
8568 |
| Tag Length |
10 |
| |
|
| Source name |
Neuroblastoma, cell line, IMR-5
|
| Organism |
Homo sapiens |
| Characteristics |
Tissue: neuroblastoma cell line IMR-5
MYCN status: amplified
|
| Biomaterial provider |
University Children's Hospital of Cologne
|
| Treatment protocol |
None
|
| Growth protocol |
Neuroblastoma cell line IMR-5 was cultured in RPMI 1640 (Biochrom KG) supplemented with 20% fetal calf serum (Biochrom KG). Cells were harvested after they were grown to confluent monolayers.
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
RNA extraction: TRIzol reagent (Invitrogen)
SAGE library construction: according to Velculescu et al. 1995, Science 270(5235):484-7
|
| |
|
| Description |
SAGE library of neuroblastoma cell line IMR-5
|
| Data processing |
SAGE2000 software, version 4.12
|
| |
|
| Submission date |
Jun 06, 2006 |
| Last update date |
Nov 19, 2008 |
| Contact name |
Matthias Fischer |
| E-mail(s) |
matthias.fischer@uk-koeln.de
|
| Phone |
0492214786816
|
| Fax |
0492214784689
|
| Organization name |
University Children's Hospital
|
| Department |
Pediatric Oncology
|
| Street address |
Kerpener Str. 62
|
| City |
Cologne |
| ZIP/Postal code |
50924 |
| Country |
Germany |
| |
|
| Platform ID |
GPL4 |
| Series (1) |
| GSE4991 |
Gene expression of disseminated neuroblastoma |
|
