|
| Status |
Public on Apr 28, 2015 |
| Title |
WT_4h_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
WT_4h
|
| Organism |
Mus musculus |
| Characteristics |
cell line: RAW264.7
strain: C57BL/6
genotye/variation: wild type
time point after lipopolysaccharide stimulus: 4 hr
|
| Treatment protocol |
IKK knockout or add p38 inhibitor pretreatment. For the conditional knocked out mice, we conditionally knocked out IKK ] (alias Ikbkb), encoding a catalytic enzyme in IkB kinase (IKK) complex, in C57BL/6 mice. IkbkbF/F (IkkbetaF/F, wild-type) and IkkbetaF/F:Mx1-Cre mice have been described in Hsu, L.C., et al., Nat Immunol, 2011. 12(2): p. 144-50. For inhibition of p38, bone marrow-derived macrophages (BMDMs) from C57BL/6 mice were treated with 10 gM SB202190 (Merck, Germany) for 2 h prior to use.
|
| Growth protocol |
The protocol was approved by the Institutional Animal Care and Use Committee of National Taiwan University College of Medicine (IACUC Approval No. 20080220). All surgery was performed under sodium pentobarbital anesthesia, and all efforts were made to minimize suffering. Bone marrow was collected from femurs and tibia of 8-10 week-old mice and used to generate bone marrow-derived macrophages (BMDMs). Briefly, bone marrow cells were collected and cultured in high glucose Dulbecco's modified Eagle's medium (DMEM) (Invitrogen, Carlsbad, CA) containing 20% L929-conditioned media for 7 days with the media replaced after 4 days to differentiate into macrophages.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from the cells were isolated using TRIzol® reagent (Invitrogen, Calsbad, CA) and purified with RNeasy® mini kits (Qiagen)
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
5125380057_C
|
| Data processing |
Quantile normalization were carried out using commercial microarray analysis software (Partek software).
|
| |
|
| Submission date |
Apr 24, 2013 |
| Last update date |
Apr 28, 2015 |
| Contact name |
Mong-Hsun Tsai |
| E-mail(s) |
motiont@gmail.com
|
| Phone |
+886-2-3366-6009
|
| Organization name |
National Taiwan University
|
| Department |
Institute of Biotechnology
|
| Street address |
4F, No. 81, Chang-Xing St.
|
| City |
Taipei |
| ZIP/Postal code |
106 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE46361 |
Transcription of Tnfaip3 is regulated by NF-kB and p38 via C/EBPβ in activated macrophages |
|
