|
| Status |
Public on Dec 31, 2013 |
| Title |
A673/TR/LOX-PP_replicate_2 |
| Sample type |
RNA |
| |
|
| Source name |
A673/TR/LOX-PP, doxycycline stimulated, 72 hours
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: A673 cell line (Ewing sarcoma)
genotype/variation: LOX-PP induced expression
|
| Treatment protocol |
Cells were stimulated with doxycycline (1 uM) for 72 hours to induce the expression of LOX-PP
|
| Growth protocol |
Cells were grown in DMEM supplemented with 10% serum and antibiotics.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using Qiagen RNeasy mini kit. Quality and integrity of the RNA was determined with a Bioanalyzer.
|
| Label |
Cy3
|
| Label protocol |
According to Agilent instructions (One-Color Microarray-based gene expression analysis v6.5)
|
| |
|
| Hybridization protocol |
According to Agilent instructions (One-Color Microarray-based gene expression analysis v6.5)
|
| Scan protocol |
According to Agilent instructions (One-Color Microarray-based gene expression analysis v6.5)
|
| Description |
LOX-PP 2
|
| Data processing |
The scanned images were analyzed with Feature Extraction Software 10.7 (Agilent) using default parameters.
|
| |
|
| Submission date |
Apr 26, 2013 |
| Last update date |
Dec 31, 2013 |
| Contact name |
Javier Alonso |
| E-mail(s) |
fjalonso@isciii.es
|
| Phone |
34-91-8223217
|
| Organization name |
INSTITUTO DE SALUD CARLOS III - IIER
|
| Department |
AREA DE GENÉTICA HUMANA
|
| Lab |
UNIDAD DE TUMORES SOLIDOS INFANTILES
|
| Street address |
CTRA. MAJADAHONDA-POZUELO KM 2
|
| City |
MAJADAHONDA |
| State/province |
MADRID |
| ZIP/Postal code |
28220 |
| Country |
Spain |
| |
|
| Platform ID |
GPL17077 |
| Series (1) |
| GSE46407 |
Effect of LOX-PP on the gene expression profile of the A673 cell line (Ewing sarcoma) |
|
