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Sample GSM1130189 Query DataSets for GSM1130189
Status Public on Oct 21, 2013
Title 11 wk B6 cerebral cortex, rep3
Sample type RNA
 
Source name 11 wk B6 cerebral cortex
Organism Mus musculus
Characteristics strain: C57BL/6J
age: post-natal 11 weeks (11 wk)
tissue: cerebral cortex
gender: male
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNA were assessed for quality with Agilent 2100 Bioanalyzer G2939A (Agilent Technologies,Santa Clara, CA)) and Nanodrop 8000 spectrophotometer (Thermo Scientific/Nanodrop, Wilmington, DE).
Label biotin
Label protocol Hybridization targets were prepared with MessageAmp™ Premier RNA Amplification Kit (Applied Biosystems/Ambion, Austin, TX) from total RNA.
 
Hybridization protocol Hybridization targets were prepared with MessageAmp™ Premier RNA Amplification Kit (Applied Biosystems/Ambion, Austin, TX) from total RNA, hybridized to GeneChip® Mouse 430 2.0 arrays in Affymetrix GeneChip® hybridization oven 645, washed in Affymetrix GeneChip® Fluidics Station 450
Scan protocol Affymetrix GeneChip® Scanner 7G according to standard Affymetrix GeneChip® Hybridization, Wash, and Stain protocols. (Affymetrix, Santa Clara,CA)
Description 11 wk B6-03
Data processing The data were analyzed with Partek Genomic Suite
 
Submission date Apr 27, 2013
Last update date Oct 21, 2013
Contact name Douglas A Marchuk
E-mail(s) douglas.marchuk@duke.edu
Organization name DUKE Univ
Street address 213 Research Dr, 277 CARL
City Durham
State/province NC
ZIP/Postal code 27705
Country USA
 
Platform ID GPL1261
Series (1)
GSE46443 Expression data from mouse cerebral cortex

Data table header descriptions
ID_REF
VALUE quantile-normalized signal in log2 scale

Data table
ID_REF VALUE
1415670_at 9.849542
1415671_at 12.3593
1415672_at 11.97607
1415673_at 8.896544
1415674_a_at 9.877973
1415675_at 9.525841
1415676_a_at 11.34623
1415677_at 9.5694
1415678_at 11.50711
1415679_at 11.07542
1415680_at 7.766154
1415681_at 10.28693
1415682_at 8.955028
1415683_at 11.28614
1415684_at 8.393075
1415685_at 9.261828
1415686_at 10.96543
1415687_a_at 12.92953
1415688_at 11.57549
1415689_s_at 9.046593

Total number of rows: 45037

Table truncated, full table size 892 Kbytes.




Supplementary file Size Download File type/resource
GSM1130189_11wk-B6-3_Mouse430+2.CEL.gz 3.7 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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