|
| Status |
Public on Oct 01, 2013 |
| Title |
4 hrs poly IC IFNAR-/- DC 2 |
| Sample type |
RNA |
| |
|
| Source name |
splenic CD11chi, IFNAR/-, poly IC, 4hr
|
| Organism |
Mus musculus |
| Characteristics |
strain/background: C57BL/6
genotype: IFNAR/-
cell type: splenic CD11chi CD3- DX5- B220- dendritic cells
treatment: poly IC
treatment duration: 4hr
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. RNA quality was assessed using the Agilent 2100 Bioanalyzer and the RNA 6000 Nano kit.
|
| Label |
biotin
|
| Label protocol |
~200 ng of total RNA was used to prepare biotin-labeled RNA using MessageAmp™ Premier RNA Amplification Kit.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol.
|
| Scan protocol |
Standard Illumina scanning protocol.
|
| Description |
SAMPLE 24
Replicate 2
|
| Data processing |
The data were normalised using quantile normalisation with GeneSpring.
|
| |
|
| Submission date |
Apr 29, 2013 |
| Last update date |
Oct 01, 2013 |
| Contact name |
Paula Longhi |
| E-mail(s) |
mlonghi@rockefeller.edu
|
| Organization name |
The Rockefeller University
|
| Street address |
1230 York Av
|
| City |
New York |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE46478 |
Transcriptional profile of WT, PRR and IFN-/- dendritic cells stimulated with poly IC in vivo |
|
