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Sample GSM1131204 Query DataSets for GSM1131204
Status Public on Apr 30, 2013
Title RefDay0_3
Sample type RNA
 
Source name RefDay0
Organism Mus musculus
Characteristics cell type: C2C12 myoblast cells
strain: C3H
Treatment protocol At 70% confluence growth medium was replaced with DMEM-HG and 2% Horse Serum +/- 100mM Ethanol. Daily medium changes.
Growth protocol C2C12 cells grown in DMEM-HG media with 20% FBS
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Data processing Robust spline normalization and base calling performed using R and analysis package lumi
 
Submission date Apr 29, 2013
Last update date Apr 30, 2013
Contact name Michelle Arya
E-mail(s) michelle.arya8@gmail.com
Organization name MCRI
Street address 15 Kneeland Street
City Boston
ZIP/Postal code 02111
Country USA
 
Platform ID GPL6885
Series (1)
GSE46492 Ethanol effect on myoblast differentiation

Data table header descriptions
ID_REF
VALUE Robust spline normalization
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1250052 67.16833333 0.40602
ILMN_3122480 44.65583333 0.25313
ILMN_2599935 663.8058333 0
ILMN_2675543 47.1475 0.12406
ILMN_2686883 40.51416667 0
ILMN_2751818 47.7975 0
ILMN_2728634 87.235 0
ILMN_3040515 51.80791667 0
ILMN_2711608 35.3975 0
ILMN_1232875 34.90166667 0.01504
ILMN_1258507 33.4475 0
ILMN_2746142 45.06833333 0
ILMN_1252690 51.00166667 0
ILMN_2655499 31.91 0
ILMN_1252870 106.8183333 0.96617
ILMN_1248179 31.66416667 0.31203
ILMN_2649955 331.6058333 0.901
ILMN_2628708 418.1266667 0
ILMN_3024781 34.21416667 0.6792
ILMN_2705628 89.22666667 0

Total number of rows: 25697

Table truncated, full table size 743 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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