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Sample GSM1131205 Query DataSets for GSM1131205
Status Public on Apr 30, 2013
Title RefDay0_4
Sample type RNA
Source name RefDay0
Organism Mus musculus
Characteristics cell type: C2C12 myoblast cells
strain: C3H
Treatment protocol At 70% confluence growth medium was replaced with DMEM-HG and 2% Horse Serum +/- 100mM Ethanol. Daily medium changes.
Growth protocol C2C12 cells grown in DMEM-HG media with 20% FBS
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Data processing Robust spline normalization and base calling performed using R and analysis package lumi
Submission date Apr 29, 2013
Last update date Apr 30, 2013
Contact name Michelle Arya
Organization name MCRI
Street address 15 Kneeland Street
City Boston
ZIP/Postal code 02111
Country USA
Platform ID GPL6885
Series (1)
GSE46492 Ethanol effect on myoblast differentiation

Data table header descriptions
VALUE Robust spline normalization
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1250052 41.18083333 0.2619
ILMN_3122480 44.18708333 0.06266
ILMN_2599935 639.4475 0
ILMN_2675543 38.135 0.12657
ILMN_2686883 46.70166667 0
ILMN_2751818 36.24125 0
ILMN_2728634 92.5975 0
ILMN_3040515 58.54333333 0
ILMN_2711608 25.56416667 0
ILMN_1232875 36.36416667 0
ILMN_1258507 44.56833333 0
ILMN_2746142 41.81625 0
ILMN_1252690 64.64125 0
ILMN_2655499 55.88708333 0
ILMN_1252870 75.47875 0.68045
ILMN_1248179 30.12666667 0.77945
ILMN_2649955 312.3225 0.63784
ILMN_2628708 458.3266667 0
ILMN_3024781 36.36416667 0.68421
ILMN_2705628 71.17666667 0

Total number of rows: 25697

Table truncated, full table size 739 Kbytes.

Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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