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Sample GSM1131213 Query DataSets for GSM1131213
Status Public on Apr 30, 2013
Title Day1_EtOH_4
Sample type RNA
 
Source name Day1_EtOH
Organism Mus musculus
Characteristics cell type: C2C12 myoblast cells
strain: C3H
Treatment protocol At 70% confluence growth medium was replaced with DMEM-HG and 2% Horse Serum +/- 100mM Ethanol. Daily medium changes.
Growth protocol C2C12 cells grown in DMEM-HG media with 20% FBS
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Data processing Robust spline normalization and base calling performed using R and analysis package lumi
 
Submission date Apr 29, 2013
Last update date Apr 30, 2013
Contact name Michelle Arya
E-mail(s) michelle.arya8@gmail.com
Organization name MCRI
Street address 15 Kneeland Street
City Boston
ZIP/Postal code 02111
Country USA
 
Platform ID GPL6885
Series (1)
GSE46492 Ethanol effect on myoblast differentiation

Data table header descriptions
ID_REF
VALUE Robust spline normalization
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1250052 50.03083333 0.41855
ILMN_3122480 21.8725 0.02005
ILMN_2599935 364.6391667 0
ILMN_2675543 53.85166667 0.54511
ILMN_2686883 76.285 0
ILMN_2751818 39.04333333 0
ILMN_2728634 112.2933333 0
ILMN_3040515 66.60166667 0
ILMN_2711608 43.185 0
ILMN_1232875 39.63291667 0.0614
ILMN_1258507 43.185 0
ILMN_2746142 39.81416667 0
ILMN_1252690 66.93083333 0
ILMN_2655499 28.49333333 0
ILMN_1252870 151.2329167 0.80451
ILMN_1248179 31.73083333 0.65789
ILMN_2649955 265.3516667 0.58772
ILMN_2628708 300.5183333 0
ILMN_3024781 44.66 0.56516
ILMN_2705628 40.92666667 0

Total number of rows: 25697

Table truncated, full table size 738 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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