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Sample GSM1131214 Query DataSets for GSM1131214
Status Public on Apr 30, 2013
Title Day2_Ctrl_1
Sample type RNA
 
Source name Day2_Ctrl
Organism Mus musculus
Characteristics cell type: C2C12 myoblast cells
strain: C3H
Treatment protocol At 70% confluence growth medium was replaced with DMEM-HG and 2% Horse Serum +/- 100mM Ethanol. Daily medium changes.
Growth protocol C2C12 cells grown in DMEM-HG media with 20% FBS
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Data processing Robust spline normalization and base calling performed using R and analysis package lumi
 
Submission date Apr 29, 2013
Last update date Apr 30, 2013
Contact name Michelle Arya
E-mail(s) michelle.arya8@gmail.com
Organization name MCRI
Street address 15 Kneeland Street
City Boston
ZIP/Postal code 02111
Country USA
 
Platform ID GPL6885
Series (1)
GSE46492 Ethanol effect on myoblast differentiation

Data table header descriptions
ID_REF
VALUE Robust spline normalization
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1250052 50.56416667 0.41353
ILMN_3122480 45.17666667 0.04887
ILMN_2599935 389.8725 0
ILMN_2675543 66.72041667 0.27068
ILMN_2686883 30.66833333 0
ILMN_2751818 29.25583333 0
ILMN_2728634 85.48083333 0
ILMN_3040515 53.70166667 0
ILMN_2711608 56.38708333 0
ILMN_1232875 34.59333333 0.43734
ILMN_1258507 19.46833333 0
ILMN_2746142 51.12666667 0
ILMN_1252690 62.79333333 0
ILMN_2655499 32.01 0
ILMN_1252870 130.81 0.94987
ILMN_1248179 33.80583333 0.82206
ILMN_2649955 246.6204167 0.54887
ILMN_2628708 260.5725 0
ILMN_3024781 45.0725 0.74561
ILMN_2705628 46.94541667 0

Total number of rows: 25697

Table truncated, full table size 740 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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