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Sample GSM1131217 Query DataSets for GSM1131217
Status Public on Apr 30, 2013
Title Day2_EtOH_1
Sample type RNA
Source name Day2_EtOH
Organism Mus musculus
Characteristics cell type: C2C12 myoblast cells
strain: C3H
Treatment protocol At 70% confluence growth medium was replaced with DMEM-HG and 2% Horse Serum +/- 100mM Ethanol. Daily medium changes.
Growth protocol C2C12 cells grown in DMEM-HG media with 20% FBS
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Data processing Robust spline normalization and base calling performed using R and analysis package lumi
Submission date Apr 29, 2013
Last update date Apr 30, 2013
Contact name Michelle Arya
Organization name MCRI
Street address 15 Kneeland Street
City Boston
ZIP/Postal code 02111
Country USA
Platform ID GPL6885
Series (1)
GSE46492 Ethanol effect on myoblast differentiation

Data table header descriptions
VALUE Robust spline normalization
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1250052 42.73916667 0.63158
ILMN_3122480 31.985 0.24937
ILMN_2599935 279.7391667 0
ILMN_2675543 33.51625 0.16291
ILMN_2686883 53.39541667 0
ILMN_2751818 25.585 0
ILMN_2728634 105.1975 0
ILMN_3040515 56.11416667 0
ILMN_2711608 38.14958333 0
ILMN_1232875 45.6975 0
ILMN_1258507 45.17666667 0
ILMN_2746142 28.31833333 0
ILMN_1252690 48.31 0
ILMN_2655499 37.36833333 0
ILMN_1252870 127.6725 0.25313
ILMN_1248179 37.50583333 0.2218
ILMN_2649955 253.5641667 0.9198
ILMN_2628708 195.2933333 0
ILMN_3024781 23.785 0.78697
ILMN_2705628 41.485 0

Total number of rows: 25697

Table truncated, full table size 738 Kbytes.

Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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