|
| Status |
Public on Apr 30, 2013 |
| Title |
Day2_EtOH_1 |
| Sample type |
RNA |
| |
|
| Source name |
Day2_EtOH
|
| Organism |
Mus musculus |
| Characteristics |
cell type: C2C12 myoblast cells
strain: C3H
|
| Treatment protocol |
At 70% confluence growth medium was replaced with DMEM-HG and 2% Horse Serum +/- 100mM Ethanol. Daily medium changes.
|
| Growth protocol |
C2C12 cells grown in DMEM-HG media with 20% FBS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Data processing |
Robust spline normalization and base calling performed using R and analysis package lumi
|
| |
|
| Submission date |
Apr 29, 2013 |
| Last update date |
Apr 30, 2013 |
| Contact name |
Michelle Arya |
| E-mail(s) |
michelle.arya8@gmail.com
|
| Organization name |
MCRI
|
| Street address |
15 Kneeland Street
|
| City |
Boston |
| ZIP/Postal code |
02111 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE46492 |
Ethanol effect on myoblast differentiation |
|
