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Sample GSM1131219 Query DataSets for GSM1131219
Status Public on Apr 30, 2013
Title Day2_EtOH_3
Sample type RNA
 
Source name Day2_EtOH
Organism Mus musculus
Characteristics cell type: C2C12 myoblast cells
strain: C3H
Treatment protocol At 70% confluence growth medium was replaced with DMEM-HG and 2% Horse Serum +/- 100mM Ethanol. Daily medium changes.
Growth protocol C2C12 cells grown in DMEM-HG media with 20% FBS
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Data processing Robust spline normalization and base calling performed using R and analysis package lumi
 
Submission date Apr 29, 2013
Last update date Apr 30, 2013
Contact name Michelle Arya
E-mail(s) michelle.arya8@gmail.com
Organization name MCRI
Street address 15 Kneeland Street
City Boston
ZIP/Postal code 02111
Country USA
 
Platform ID GPL6885
Series (1)
GSE46492 Ethanol effect on myoblast differentiation

Data table header descriptions
ID_REF
VALUE Robust spline normalization
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1250052 59.74333333 0.97118
ILMN_3122480 26.60375 0.16416
ILMN_2599935 273.7766667 0.00251
ILMN_2675543 49.2475 0.01378
ILMN_2686883 51.02458333 0
ILMN_2751818 32.50166667 0
ILMN_2728634 96.25166667 0
ILMN_3040515 75.685 0
ILMN_2711608 47.0725 0
ILMN_1232875 58.32666667 0.04637
ILMN_1258507 33.15166667 0
ILMN_2746142 37.91833333 0
ILMN_1252690 47.0725 0
ILMN_2655499 40.585 0
ILMN_1252870 121.2995833 0.6203
ILMN_1248179 45.91833333 0.9812
ILMN_2649955 256.7433333 0.02757
ILMN_2628708 256.3558333 0
ILMN_3024781 43.53916667 0.88346
ILMN_2705628 56.8725 0.00251

Total number of rows: 25697

Table truncated, full table size 745 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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