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Sample GSM1131220 Query DataSets for GSM1131220
Status Public on Apr 30, 2013
Title Day3_Ctrl_1
Sample type RNA
Source name Day3_Ctrl
Organism Mus musculus
Characteristics cell type: C2C12 myoblast cells
strain: C3H
Treatment protocol At 70% confluence growth medium was replaced with DMEM-HG and 2% Horse Serum +/- 100mM Ethanol. Daily medium changes.
Growth protocol C2C12 cells grown in DMEM-HG media with 20% FBS
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Data processing Robust spline normalization and base calling performed using R and analysis package lumi
Submission date Apr 29, 2013
Last update date Apr 30, 2013
Contact name Michelle Arya
Organization name MCRI
Street address 15 Kneeland Street
City Boston
ZIP/Postal code 02111
Country USA
Platform ID GPL6885
Series (1)
GSE46492 Ethanol effect on myoblast differentiation

Data table header descriptions
VALUE Robust spline normalization
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1250052 75.49333333 0.71178
ILMN_3122480 41.25583333 0.09524
ILMN_2599935 304.5370833 0.00125
ILMN_2675543 36.3975 0.14286
ILMN_2686883 50.87041667 0
ILMN_2751818 51.60166667 0
ILMN_2728634 66.78916667 0
ILMN_3040515 57.65583333 0
ILMN_2711608 16.65583333 0
ILMN_1232875 34.11416667 0.03383
ILMN_1258507 31.04333333 0
ILMN_2746142 42.30791667 0
ILMN_1252690 57.75166667 0
ILMN_2655499 45.1725 0
ILMN_1252870 129.0954167 0.26942
ILMN_1248179 19.76625 0.01378
ILMN_2649955 221.86 0.80451
ILMN_2628708 207.7766667 0
ILMN_3024781 53.32666667 0.95363
ILMN_2705628 37.14333333 0.00125

Total number of rows: 25697

Table truncated, full table size 743 Kbytes.

Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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