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Sample GSM1131221 Query DataSets for GSM1131221
Status Public on Apr 30, 2013
Title Day3_Ctrl_2
Sample type RNA
Source name Day3_Ctrl
Organism Mus musculus
Characteristics cell type: C2C12 myoblast cells
strain: C3H
Treatment protocol At 70% confluence growth medium was replaced with DMEM-HG and 2% Horse Serum +/- 100mM Ethanol. Daily medium changes.
Growth protocol C2C12 cells grown in DMEM-HG media with 20% FBS
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Data processing Robust spline normalization and base calling performed using R and analysis package lumi
Submission date Apr 29, 2013
Last update date Apr 30, 2013
Contact name Michelle Arya
Organization name MCRI
Street address 15 Kneeland Street
City Boston
ZIP/Postal code 02111
Country USA
Platform ID GPL6885
Series (1)
GSE46492 Ethanol effect on myoblast differentiation

Data table header descriptions
VALUE Robust spline normalization
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1250052 59.985 0.90602
ILMN_3122480 32.44125 0.0188
ILMN_2599935 407.3766667 0
ILMN_2675543 33.435 0.26942
ILMN_2686883 38.96 0
ILMN_2751818 24.84333333 0
ILMN_2728634 76.95583333 0
ILMN_3040515 44.12666667 0
ILMN_2711608 45.70166667 0
ILMN_1232875 25.94333333 0.03509
ILMN_1258507 49.73916667 0
ILMN_2746142 50.41833333 0
ILMN_1252690 59.03916667 0
ILMN_2655499 39.7975 0
ILMN_1252870 124.935 0.74185
ILMN_1248179 39.33916667 0.87093
ILMN_2649955 164.3933333 0.51629
ILMN_2628708 218.2808333 0
ILMN_3024781 32.01 0.7218
ILMN_2705628 31.34333333 0

Total number of rows: 25697

Table truncated, full table size 740 Kbytes.

Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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