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Sample GSM1131222 Query DataSets for GSM1131222
Status Public on Apr 30, 2013
Title Day3_Ctrl_3
Sample type RNA
Source name Day3_Ctrl
Organism Mus musculus
Characteristics cell type: C2C12 myoblast cells
strain: C3H
Treatment protocol At 70% confluence growth medium was replaced with DMEM-HG and 2% Horse Serum +/- 100mM Ethanol. Daily medium changes.
Growth protocol C2C12 cells grown in DMEM-HG media with 20% FBS
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Data processing Robust spline normalization and base calling performed using R and analysis package lumi
Submission date Apr 29, 2013
Last update date Apr 30, 2013
Contact name Michelle Arya
Organization name MCRI
Street address 15 Kneeland Street
City Boston
ZIP/Postal code 02111
Country USA
Platform ID GPL6885
Series (1)
GSE46492 Ethanol effect on myoblast differentiation

Data table header descriptions
VALUE Robust spline normalization
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1250052 40.71 0.45865
ILMN_3122480 40.36 0.07268
ILMN_2599935 379.8475 0.00125
ILMN_2675543 37.21 0.07769
ILMN_2686883 35.00583333 0
ILMN_2751818 22.80166667 0
ILMN_2728634 81.21416667 0
ILMN_3040515 35.68083333 0
ILMN_2711608 31.6975 0
ILMN_1232875 39.735 0.02506
ILMN_1258507 46.085 0
ILMN_2746142 31.78916667 0
ILMN_1252690 45.17666667 0
ILMN_2655499 30.31625 0
ILMN_1252870 131.10375 0.95614
ILMN_1248179 21.52666667 0.32581
ILMN_2649955 213.135 0.14536
ILMN_2628708 177.0141667 0
ILMN_3024781 39.21416667 0.85589
ILMN_2705628 27.89333333 0.00251

Total number of rows: 25697

Table truncated, full table size 741 Kbytes.

Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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