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Sample GSM1131225 Query DataSets for GSM1131225
Status Public on Apr 30, 2013
Title Day3_EtOH_3
Sample type RNA
 
Source name Day3_EtOH
Organism Mus musculus
Characteristics cell type: C2C12 myoblast cells
strain: C3H
Treatment protocol At 70% confluence growth medium was replaced with DMEM-HG and 2% Horse Serum +/- 100mM Ethanol. Daily medium changes.
Growth protocol C2C12 cells grown in DMEM-HG media with 20% FBS
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Data processing Robust spline normalization and base calling performed using R and analysis package lumi
 
Submission date Apr 29, 2013
Last update date Apr 30, 2013
Contact name Michelle Arya
E-mail(s) michelle.arya8@gmail.com
Organization name MCRI
Street address 15 Kneeland Street
City Boston
ZIP/Postal code 02111
Country USA
 
Platform ID GPL6885
Series (1)
GSE46492 Ethanol effect on myoblast differentiation

Data table header descriptions
ID_REF
VALUE Robust spline normalization
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1250052 43.02875 0.80576
ILMN_3122480 30.93083333 0.02506
ILMN_2599935 372.4433333 0
ILMN_2675543 56.48083333 0.82331
ILMN_2686883 44.31416667 0
ILMN_2751818 36.3225 0
ILMN_2728634 87.20166667 0
ILMN_3040515 61.40583333 0
ILMN_2711608 52.48916667 0
ILMN_1232875 44.65583333 0.02632
ILMN_1258507 44.7725 0
ILMN_2746142 22.0725 0
ILMN_1252690 35.4725 0
ILMN_2655499 32.00583333 0
ILMN_1252870 161.27875 0.32206
ILMN_1248179 36.735 0.04887
ILMN_2649955 223.1141667 0.62281
ILMN_2628708 194.8204167 0
ILMN_3024781 45.36416667 0.41103
ILMN_2705628 23.7975 0.01253

Total number of rows: 25697

Table truncated, full table size 744 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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