|
| Status |
Public on Oct 02, 2013 |
| Title |
MII stage oocyte, wild type, biological rep 3 |
| Sample type |
RNA |
| |
|
| Source name |
polysome bound transcripts from mouse MII-stage oocytes, wild type
|
| Organism |
Mus musculus |
| Characteristics |
tissue: oocyte
Stage: MII
strain: C57BL/6J 129Sv
background: wild type
|
| Treatment protocol |
Forty-eight hours (h) after PMSG injection, mice were stimulated with hCG for 0, or 14 h, and GV and MII stage oocytes were collected.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Polysome bound mRNAs were purified using Rneasy Plus Micro kit (Qiagen), and then reverse-transcribed and linearly amplified with WT-Ovation FFPE RNA Amplification System V2 (NuGEN).
|
| Label |
biotin
|
| Label protocol |
cDNA was fragment and labeled using NuGEN WT-Ovation FFPE System & Encore Biotin Module
|
| |
|
| Hybridization protocol |
Hybridization was followed by GeneChip Expression Analysis Technical Manual 702232 Rev. 2
|
| Scan protocol |
GeneChips were scanned using the the Affymetrix GCS3000 microarray system
|
| Description |
Gene expression data from embryos in slow phase of cellularisation.
|
| Data processing |
Microarray data were normalized using the Robust Multi-array Average (RMA) method. RMA was performed using the affy library in Bioconductor and a custom chip definition file (CDF) representing the latest RefSeq probe mapping to the mouse genome (Mouse4302_Mm_REFSEQ, Version 16.0.0), downloaded from the Microarray Lab of the Molecular and Behavioral Neuroscience Institute, University of Michigan.
|
| |
|
| Submission date |
May 03, 2013 |
| Last update date |
Oct 02, 2013 |
| Contact name |
Courtney Onodera |
| E-mail(s) |
courtney.onodera@ucsf.edu
|
| Organization name |
University of California, San Francisco
|
| Street address |
1450 Third St, HD374
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94158 |
| Country |
USA |
| |
|
| Platform ID |
GPL17114 |
| Series (1) |
| GSE46640 |
Comparison of the translational program in oocytes from wild type and Areg KO mice |
|
