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Sample GSM1138096 Query DataSets for GSM1138096
Status Public on Jan 21, 2014
Title P4KO rescued with PMCA4a replicate 3
Sample type RNA
 
Source name carotid artery; male mouse
Organism Mus musculus
Characteristics cell type: primary vascular smooth muscle cells
genotype: PMCA4 knockout rescued with PMCA4a
genetic background: C57bl6
gender: male
cell cycle stage: late G1/S stage
Treatment protocol Cells were stimulated with serum and PDGF-BB and harvested at the late G1/S stage of the cell cycle.
Growth protocol P4KO primary VSMC were serum synchronized. Alternatively, P4KO cells electroporated with PMCA4a cDNA were serum synchronized.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted with Life Technologies PicoPure RNA extraction kit and quantified by Nanodrop.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion Illumina Total Prep RNA Amplification Kit Cat.# IL 1791 for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description P4KO+4a-#3
Data processing The data were normalised using quantile normalisation with lumi in R
 
Submission date May 08, 2013
Last update date Jan 21, 2014
Contact name Mansoor Husain
E-mail(s) mansoor.husain@uhn.ca
Phone 416-581-7489
Organization name University Health Network
Department Toronto Gen. Hosp. Res Institute
Lab Mansoor Husain lab
Street address Room TMDT-3-910, 101 College St.
City Toronto
State/province Ontario
ZIP/Postal code M5G 1L7
Country Canada
 
Platform ID GPL6885
Series (2)
GSE38320 Regulated Splicing of Plasma Membrane Ca2+ ATPase-4 (PMCA4) Controls Cell Cycle Progression in Vascular Smooth Muscle Cells
GSE46744 Genome wide analysis of genes expressed in late G1/S phase of the cell cycle in PMCA4 knockout (P4KO) and P4KO rescued with PMCA4a primary vascular smooth muscle cells

Data table header descriptions
ID_REF
VALUE quantile normalized
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_2896528 12.74465786 0
ILMN_2721178 10.78101151 0
ILMN_3033922 11.13994284 0
ILMN_3092673 13.17114964 0
ILMN_2816356 6.025886466 0.002506266
ILMN_2808939 10.28374922 0
ILMN_2634564 9.69414877 0
ILMN_2737647 4.789804808 0.2192982
ILMN_2734484 7.869483765 0
ILMN_2952292 7.275669358 0
ILMN_2699078 4.600748549 0.5275689
ILMN_1213681 9.149240864 0
ILMN_2735413 4.63128409 0.4473684
ILMN_2735415 4.711863836 0.3107769
ILMN_2891688 10.10244525 0
ILMN_2637698 11.0653075 0
ILMN_2674228 8.734523492 0
ILMN_2601546 4.853715417 0.1716792
ILMN_1230831 4.581648673 0.5776942
ILMN_2848071 5.478565399 0.02130326

Total number of rows: 25697

Table truncated, full table size 804 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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