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Sample GSM1138098 Query DataSets for GSM1138098
Status Public on Jan 21, 2014
Title PMCA4 knockout replicate 1
Sample type RNA
Source name carotid artery; male mouse
Organism Mus musculus
Characteristics cell type: primary vascular smooth muscle cells
genotype: PMCA4 knockout
genetic background: C57bl6
gender: male
cell cycle stage: late G1/S stage
Treatment protocol Cells were stimulated with serum and PDGF-BB and harvested at the late G1/S stage of the cell cycle.
Growth protocol P4KO primary VSMC were serum synchronized. Alternatively, P4KO cells electroporated with PMCA4a cDNA were serum synchronized.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted with Life Technologies PicoPure RNA extraction kit and quantified by Nanodrop.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion Illumina Total Prep RNA Amplification Kit Cat.# IL 1791 for Illumina arrays
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description P4KO#1
Data processing The data were normalised using quantile normalisation with lumi in R
Submission date May 08, 2013
Last update date Jan 21, 2014
Contact name Mansoor Husain
Phone 416-581-7489
Organization name University Health Network
Department Toronto Gen. Hosp. Res Institute
Lab Mansoor Husain lab
Street address Room TMDT-3-910, 101 College St.
City Toronto
State/province Ontario
ZIP/Postal code M5G 1L7
Country Canada
Platform ID GPL6885
Series (2)
GSE38320 Regulated Splicing of Plasma Membrane Ca2+ ATPase-4 (PMCA4) Controls Cell Cycle Progression in Vascular Smooth Muscle Cells
GSE46744 Genome wide analysis of genes expressed in late G1/S phase of the cell cycle in PMCA4 knockout (P4KO) and P4KO rescued with PMCA4a primary vascular smooth muscle cells

Data table header descriptions
VALUE quantile normalized
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_2896528 12.6943882 0
ILMN_2721178 10.69960305 0
ILMN_3033922 10.77332161 0
ILMN_3092673 12.93333112 0
ILMN_2816356 5.865066729 0.006265664
ILMN_2808939 11.00994897 0
ILMN_2634564 10.08487815 0
ILMN_2737647 4.639314103 0.4197995
ILMN_2734484 7.844375725 0
ILMN_2952292 7.585831 0
ILMN_2699078 4.60694376 0.5037594
ILMN_1213681 8.687300899 0
ILMN_2735413 4.583865709 0.5676692
ILMN_2735415 4.746216542 0.2506266
ILMN_2891688 9.501679014 0
ILMN_2637698 11.22633776 0
ILMN_2674228 8.865293997 0
ILMN_2601546 4.845170896 0.1842105
ILMN_1230831 4.627174225 0.4486215
ILMN_2848071 5.610013709 0.01879699

Total number of rows: 25697

Table truncated, full table size 810 Kbytes.

Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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