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Sample GSM1138448 Query DataSets for GSM1138448
Status Public on Jun 09, 2013
Title NSC_1
Sample type RNA
 
Source name mESC derived NSC
Organism Mus musculus
Characteristics strain: C57BL/6
cell type: neural stem cellsderived from mouse embryonic stem cells
tau-redstar expression: No
cell type: neural stem cellsderived from mouse embryonic stem cells
Treatment protocol Differentiation to neurons was performed in N2B27 for four days, followed by a replating step in Neurobasal medium supplemented with B27. Neurons were then FACS purified based on RedStar expression driven by the neuronal specific Tau promoter
Growth protocol NSCs were derived according to monolayer protocols (Ying et al 2003) and maintained in Euromed-N medium supplemented with N2 and FGF2/EGF.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using the Qiagen RNA easy columns according to manufacturers protocols
Label Biotin
Label protocol RNA was labelled using a TotalPrep RNA Amplification Kit (Ambion).
 
Hybridization protocol Standard Illumina hybridization protocol to Illumina Mouse Ref-8 v2 BeadArrays.
Scan protocol Standard Illumina scanning protocol.
Description 4639127051_A
Data processing The data were normalized using quantile normalization using the Bioconductor BeadArray library in R. Differential expression was calculated using the limma library.
 
Submission date May 09, 2013
Last update date Jun 09, 2013
Contact name Matt Burney
Organization name King's College London
Street address 125 Coldharbour Lane
City London
ZIP/Postal code SE5 9NU
Country United Kingdom
 
Platform ID GPL6885
Series (2)
GSE46791 An epigenetic signature of developmental potential in neural stem cells and early neurons [expression]
GSE46793 An epigenetic signature of developmental potential in neural stem cells and early neurons

Data table header descriptions
ID_REF
VALUE log2 quantile-normalized signal intensity

Data table
ID_REF VALUE
ILMN_1250052 6.421782817
ILMN_3122480 6.104632443
ILMN_2599935 8.686700366
ILMN_2675543 6.400806656
ILMN_2686883 6.27167074
ILMN_2751818 6.158876445
ILMN_2728634 6.614687762
ILMN_3040515 6.426383505
ILMN_2711608 6.354185013
ILMN_1232875 6.247913959
ILMN_1258507 6.302598244
ILMN_2746142 5.942572831
ILMN_1253709 6.22665194
ILMN_1231129 10.77655705
ILMN_2711788 6.104632443
ILMN_2707099 5.913549262
ILMN_2942000 6.582089872
ILMN_2642403 12.11757646
ILMN_2935636 6.339350963
ILMN_1242024 6.350436903

Total number of rows: 25697

Table truncated, full table size 623 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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