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Sample GSM1138449 Query DataSets for GSM1138449
Status Public on Jun 09, 2013
Title NSC_2
Sample type RNA
 
Source name mESC derived NSC
Organism Mus musculus
Characteristics strain: C57BL/6
cell type: neural stem cellsderived from mouse embryonic stem cells
tau-redstar expression: No
cell type: neural stem cellsderived from mouse embryonic stem cells
Treatment protocol Differentiation to neurons was performed in N2B27 for four days, followed by a replating step in Neurobasal medium supplemented with B27. Neurons were then FACS purified based on RedStar expression driven by the neuronal specific Tau promoter
Growth protocol NSCs were derived according to monolayer protocols (Ying et al 2003) and maintained in Euromed-N medium supplemented with N2 and FGF2/EGF.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using the Qiagen RNA easy columns according to manufacturers protocols
Label Biotin
Label protocol RNA was labelled using a TotalPrep RNA Amplification Kit (Ambion).
 
Hybridization protocol Standard Illumina hybridization protocol to Illumina Mouse Ref-8 v2 BeadArrays.
Scan protocol Standard Illumina scanning protocol.
Description 4639127051_B
Data processing The data were normalized using quantile normalization using the Bioconductor BeadArray library in R. Differential expression was calculated using the limma library.
 
Submission date May 09, 2013
Last update date Jun 09, 2013
Contact name Matt Burney
Organization name King's College London
Street address 125 Coldharbour Lane
City London
ZIP/Postal code SE5 9NU
Country United Kingdom
 
Platform ID GPL6885
Series (2)
GSE46791 An epigenetic signature of developmental potential in neural stem cells and early neurons [expression]
GSE46793 An epigenetic signature of developmental potential in neural stem cells and early neurons

Data table header descriptions
ID_REF
VALUE log2 quantile-normalized signal intensity

Data table
ID_REF VALUE
ILMN_1250052 6.365603492
ILMN_3122480 6.028786838
ILMN_2599935 8.845053459
ILMN_2675543 6.284903032
ILMN_2686883 6.339791618
ILMN_2751818 6.199916827
ILMN_2728634 6.630154282
ILMN_3040515 6.216503633
ILMN_2711608 6.373232597
ILMN_1232875 6.111429253
ILMN_1258507 6.363852496
ILMN_2746142 6.056150407
ILMN_1253709 6.076849528
ILMN_1231129 10.88945615
ILMN_2711788 6.46645142
ILMN_2707099 6.035269068
ILMN_2942000 6.39846295
ILMN_2642403 11.77874007
ILMN_2935636 6.374750303
ILMN_1242024 6.549920986

Total number of rows: 25697

Table truncated, full table size 624 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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