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Sample GSM1138453 Query DataSets for GSM1138453
Status Public on Jun 09, 2013
Title Neuron_1
Sample type RNA
 
Source name NSC derived Neuron
Organism Mus musculus
Characteristics strain: C57BL/6
cell type: differentiated neuronal progeny
tau-redstar expression: Yes
cell type: differentiated neuronal progeny
Treatment protocol Differentiation to neurons was performed in N2B27 for four days, followed by a replating step in Neurobasal medium supplemented with B27. Neurons were then FACS purified based on RedStar expression driven by the neuronal specific Tau promoter
Growth protocol NSCs were derived according to monolayer protocols (Ying et al 2003) and maintained in Euromed-N medium supplemented with N2 and FGF2/EGF.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using the Qiagen RNA easy columns according to manufacturers protocols
Label Biotin
Label protocol RNA was labelled using a TotalPrep RNA Amplification Kit (Ambion).
 
Hybridization protocol Standard Illumina hybridization protocol to Illumina Mouse Ref-8 v2 BeadArrays.
Scan protocol Standard Illumina scanning protocol.
Description 4639127051_F
Data processing The data were normalized using quantile normalization using the Bioconductor BeadArray library in R. Differential expression was calculated using the limma library.
 
Submission date May 09, 2013
Last update date Jun 09, 2013
Contact name Matt Burney
Organization name King's College London
Street address 125 Coldharbour Lane
City London
ZIP/Postal code SE5 9NU
Country United Kingdom
 
Platform ID GPL6885
Series (2)
GSE46791 An epigenetic signature of developmental potential in neural stem cells and early neurons [expression]
GSE46793 An epigenetic signature of developmental potential in neural stem cells and early neurons

Data table header descriptions
ID_REF
VALUE log2 quantile-normalized signal intensity

Data table
ID_REF VALUE
ILMN_1250052 6.318044762
ILMN_3122480 6.364065283
ILMN_2599935 8.844304467
ILMN_2675543 6.080581643
ILMN_2686883 6.175435208
ILMN_2751818 6.179168745
ILMN_2728634 6.68065713
ILMN_3040515 6.415665821
ILMN_2711608 6.319398074
ILMN_1232875 6.432759219
ILMN_1258507 6.280514377
ILMN_2746142 6.002872044
ILMN_1253709 6.211643077
ILMN_1231129 11.13864914
ILMN_2711788 6.169433432
ILMN_2707099 6.143393228
ILMN_2942000 6.637777784
ILMN_2642403 11.812264
ILMN_2935636 6.312840763
ILMN_1242024 6.336442355

Total number of rows: 25697

Table truncated, full table size 623 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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