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Sample GSM1138454 Query DataSets for GSM1138454
Status Public on Jun 09, 2013
Title Neuron_2
Sample type RNA
 
Source name NSC derived Neuron
Organism Mus musculus
Characteristics strain: C57BL/6
cell type: differentiated neuronal progeny
tau-redstar expression: Yes
cell type: differentiated neuronal progeny
Treatment protocol Differentiation to neurons was performed in N2B27 for four days, followed by a replating step in Neurobasal medium supplemented with B27. Neurons were then FACS purified based on RedStar expression driven by the neuronal specific Tau promoter
Growth protocol NSCs were derived according to monolayer protocols (Ying et al 2003) and maintained in Euromed-N medium supplemented with N2 and FGF2/EGF.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using the Qiagen RNA easy columns according to manufacturers protocols
Label Biotin
Label protocol RNA was labelled using a TotalPrep RNA Amplification Kit (Ambion).
 
Hybridization protocol Standard Illumina hybridization protocol to Illumina Mouse Ref-8 v2 BeadArrays.
Scan protocol Standard Illumina scanning protocol.
Description 4639127051_G
Data processing The data were normalized using quantile normalization using the Bioconductor BeadArray library in R. Differential expression was calculated using the limma library.
 
Submission date May 09, 2013
Last update date Jun 09, 2013
Contact name Matt Burney
Organization name King's College London
Street address 125 Coldharbour Lane
City London
ZIP/Postal code SE5 9NU
Country United Kingdom
 
Platform ID GPL6885
Series (2)
GSE46791 An epigenetic signature of developmental potential in neural stem cells and early neurons [expression]
GSE46793 An epigenetic signature of developmental potential in neural stem cells and early neurons

Data table header descriptions
ID_REF
VALUE log2 quantile-normalized signal intensity

Data table
ID_REF VALUE
ILMN_1250052 6.411230198
ILMN_3122480 6.108295633
ILMN_2599935 8.574839885
ILMN_2675543 6.314885777
ILMN_2686883 6.012676655
ILMN_2751818 6.202250013
ILMN_2728634 6.727189594
ILMN_3040515 6.290675797
ILMN_2711608 6.304884676
ILMN_1232875 6.1145566
ILMN_1258507 6.311937703
ILMN_2746142 6.1145566
ILMN_1253709 6.08589839
ILMN_1231129 11.22569401
ILMN_2711788 6.270271058
ILMN_2707099 6.207628055
ILMN_2942000 6.605472536
ILMN_2642403 11.58985057
ILMN_2935636 6.413974571
ILMN_1242024 6.169433432

Total number of rows: 25697

Table truncated, full table size 624 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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