|
| Status |
Public on May 11, 2013 |
| Title |
Multiciliated cell_control_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Multiciliated cell from embryos injected with control morpholino
|
| Organism |
Danio rerio |
| Characteristics |
strain/background: AB
genotype/variation: Tg(cmyb:EGFP)
cell type: kidney multiciliated cells
developmental stage: 3 days post fertilization embryo
morpholino: control
|
| Treatment protocol |
Tissue block containing the kidney was dissected from 3 days post fertilization Tg(cmyb:EGFP) embryos, digested with 0.25% trypsin (Invitrogen) for 1hour and filtered through 40 um cell strainer (BD Falcon). 20000 GFP+ cells were sorted out using FACS Aria flow cytometer (BD) and used for RNA extraction.
|
| Growth protocol |
Zebrafish embryos were raised under 28.5 degrees.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions. 100 ng total RNA was amplified using Ovation RNA Amplification System.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2003, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 5 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Zebrafish Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using GeneChip Scanner 3000 (P/N 00-00212, Affymetrix).
|
| Description |
Gene expression data of kidney multiciliated cells from embryos injected with control morpholino.
|
| Data processing |
The data were analyzed with Command Console Software 3.1 using Affymetrix default analysis settings. Primary data was normalized by GeneSpring Software 11.0 (Agilent) using MAS5.0 as normalization method.
|
| |
|
| Submission date |
May 10, 2013 |
| Last update date |
Jul 30, 2013 |
| Contact name |
Lei Wang |
| E-mail(s) |
wangleigoal@gmail.com
|
| Organization name |
Institute of Health Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences
|
| Street address |
Room 408, Building 1 225 South Chong Qing Road
|
| City |
Shanghai |
| ZIP/Postal code |
200025 |
| Country |
China |
| |
|
| Platform ID |
GPL1319 |
| Series (1) |
| GSE46844 |
Expression data of kidney multiciliated cells from zebrafish embryos |
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