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Sample GSM1140691 Query DataSets for GSM1140691
Status Public on Feb 13, 2014
Title M_GMCSF_TPP_IFNb_IFNg_72h [5376074038_C]
Sample type RNA
 
Source name peripheral blood
Organism Homo sapiens
Characteristics initial differentiation: GM-CSF
activation stimuli: IFNb 100U/ml + TNFa 800 u/ml+ PGE2 1µg/ml+ P3C 1µg/ml + IFNg 200U/ml
time: 72h
donor: BC88
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description Macrophage
Data processing The data were log2-transformed and normalized using quantile normalization with Partek Genomics Suite
 
Submission date May 14, 2013
Last update date Feb 13, 2014
Contact name Joachim Schultze
E-mail(s) j.schultze@uni-bonn.de
Organization name LIMES (Life and Medical Sciences Center Genomics and Immunoregulation)
Department Genomics and Immunoregulation
Street address Carl-Troll-Strasse 31
City Bonn
State/province NRW
ZIP/Postal code 53115
Country Germany
 
Platform ID GPL6947
Series (2)
GSE46903 Transcriptome-based network analysis reveals a spectrum model of human macrophage activation [Expression]
GSE47189 Transcriptome-based network analysis reveals a spectrum model of human macrophage activation

Data table header descriptions
ID_REF
VALUE log2-transformed,quantile normalized
5376074038_C.Detection Pval

Data table
ID_REF VALUE 5376074038_C.Detection Pval
ILMN_1762337 6.32944 0.466403
ILMN_2055271 6.46014 0.139657
ILMN_1736007 6.45499 0.150198
ILMN_2383229 6.37797 0.31357
ILMN_1806310 6.33714 0.44137
ILMN_1779670 6.62786 0.0316206
ILMN_1653355 6.54467 0.057971
ILMN_1717783 6.27242 0.703557
ILMN_1705025 6.34494 0.41502
ILMN_1814316 6.1752 0.905138
ILMN_2359168 6.24519 0.782609
ILMN_1731507 6.25681 0.748353
ILMN_1787689 6.50633 0.0895916
ILMN_1745607 6.49325 0.0974967
ILMN_2136495 6.51037 0.0843215
ILMN_1668111 6.34243 0.422925
ILMN_2295559 6.34786 0.403162
ILMN_1735045 7.48015 0
ILMN_1680754 6.3662 0.354414
ILMN_2375184 6.27328 0.699605

Total number of rows: 47463

Table truncated, full table size 1335 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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