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Sample GSM1140692 Query DataSets for GSM1140692
Status Public on Feb 13, 2014
Title M_GMCSF_TPP_IFNb_IFNg_72h [5376074038_D]
Sample type RNA
 
Source name peripheral blood
Organism Homo sapiens
Characteristics initial differentiation: GM-CSF
activation stimuli: IFNb 100U/ml + TNFa 800 u/ml+ PGE2 1µg/ml+ P3C 1µg/ml + IFNg 200U/ml
time: 72h
donor: BC89
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description Macrophage
Data processing The data were log2-transformed and normalized using quantile normalization with Partek Genomics Suite
 
Submission date May 14, 2013
Last update date Feb 13, 2014
Contact name Joachim Schultze
E-mail(s) j.schultze@uni-bonn.de
Organization name LIMES (Life and Medical Sciences Center Genomics and Immunoregulation)
Department Genomics and Immunoregulation
Street address Carl-Troll-Strasse 31
City Bonn
State/province NRW
ZIP/Postal code 53115
Country Germany
 
Platform ID GPL6947
Series (2)
GSE46903 Transcriptome-based network analysis reveals a spectrum model of human macrophage activation [Expression]
GSE47189 Transcriptome-based network analysis reveals a spectrum model of human macrophage activation

Data table header descriptions
ID_REF
VALUE log2-transformed,quantile normalized
5376074038_D.Detection Pval

Data table
ID_REF VALUE 5376074038_D.Detection Pval
ILMN_1762337 6.21899 0.843215
ILMN_2055271 6.4614 0.126482
ILMN_1736007 6.28923 0.615283
ILMN_2383229 6.2981 0.58498
ILMN_1806310 6.25862 0.706192
ILMN_1779670 6.79744 0.0131752
ILMN_1653355 6.89026 0.0105402
ILMN_1717783 6.22238 0.837945
ILMN_1705025 6.82122 0.0131752
ILMN_1814316 6.23441 0.793149
ILMN_2359168 6.56277 0.0500659
ILMN_1731507 6.25043 0.744401
ILMN_1787689 6.35486 0.380764
ILMN_1745607 6.21481 0.855072
ILMN_2136495 6.4304 0.192358
ILMN_1668111 6.38734 0.28722
ILMN_2295559 6.32575 0.467721
ILMN_1735045 7.01647 0.00263505
ILMN_1680754 6.29689 0.59025
ILMN_2375184 6.30163 0.578393

Total number of rows: 47463

Table truncated, full table size 1343 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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