|
| Status |
Public on Sep 01, 2013 |
| Title |
NSN-CCs-2 |
| Sample type |
RNA |
| |
|
| Source name |
cumulus cells (CCs) from antral mouse oocytes of known developmental incompetence
|
| Organism |
Mus musculus |
| Characteristics |
strain: B6C3F1
gender: female
age: six weeks
tissue: oocytes
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The RNA was isolated using RNeasy Mini Kit (Quiagen), and DNase digestion (RNase-free DNase Set, Quiagen) has been applied on the columns during isolation.
|
| Label |
biotin
|
| Label protocol |
Illumina Gene Expression protocols contain a first- and second-strand reverse transcription step, followed by a single in vitro transcription (IVT) amplification that incorporates biotin-labeled nucleotides. Subsequent steps include array hybridization, washing, blocking, and streptavadin-Cy3 staining. Fluorescence emission by Cy3 is quantitatively detected for downstream analysis.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 2
|
| Data processing |
Rank invariant normalization using Illumina BeadStudio software version 3
|
| |
|
| Submission date |
May 14, 2013 |
| Last update date |
Sep 01, 2013 |
| Contact name |
Alessandro Prigione |
| E-mail(s) |
alessandro.prigione@mdc-berlin.de
|
| Organization name |
Max Delbrueck Center for Molecular Medicine
|
| Lab |
Department of Neuroproteomics
|
| Street address |
Robert-Roessle-Str. 10
|
| City |
Berlin |
| State/province |
Berlin |
| ZIP/Postal code |
D-13125 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE46906 |
Transcriptome based identification of mouse cumulus cell markers that predict the developmental competence of their enclosed antral oocytes |
|
