|
| Status |
Public on May 21, 2013 |
| Title |
CuCPF22_21June07 |
| Sample type |
RNA |
| |
|
| Source name |
olfactory rosettes
|
| Organism |
Danio rerio |
| Characteristics |
treatment: CPF= 0.25 , Cu= 0.25
tissue: olfactory rosettes, olfactory bulbs, and telencephalons
|
| Biomaterial provider |
The University of Washington (Seattle, WA).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extraction was performed by the CEEH Genomics Core Laboratory followed by the manufacturer’s protocols using the GeneChip platform by Affymetrix.
|
| Label |
biotin
|
| Label protocol |
For microarray hybridization, RNA was processed for hybridization to Affymetrix Zebrafish Arrays through a core facility in the Functional Genomics Laboratory in the Center for Ecogenetics and Environmental Health at the University of Washington according to the manufacturer's instructions.
|
| |
|
| Hybridization protocol |
The standard hybridization protocol was used as recommended by Affymterix.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneArray Scanner 3000 through the University of Washington Functional Genomics Laboratory in the Center for Ecogenetics and Environmental Health (http://depts.washington.edu/ceeh/ServiceCores/FC1/FC1.html).
|
| Description |
olfactory rosettes
|
| Data processing |
Bioconductor, GCRMA normalization log2
|
| |
|
| Submission date |
May 16, 2013 |
| Last update date |
Sep 05, 2017 |
| Contact name |
James William MacDonald |
| E-mail(s) |
jmacdon@uw.edu
|
| Organization name |
University of Washington
|
| Department |
Environmental and Occupational Health Sciences
|
| Street address |
4225 Roosevelt Way NE
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98105-6099 |
| Country |
USA |
| |
|
| Platform ID |
GPL1319 |
| Series (1) |
| GSE47039 |
Transcriptional impact of organophosphate and metal mixtures on olfaction: copper dominates the chlorpyrifos-induced response in adult zebrafish. |
|
