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Sample GSM1143941 Query DataSets for GSM1143941
Status Public on May 13, 2014
Title ESC rep 1
Sample type RNA
Source name ESC
Organism Mus musculus
Characteristics cell type: mouse embryonic stem cell
treated: No
Extracted molecule total RNA
Extraction protocol 500 ng of total DNA-free RNA was used as input for labelled cRNA synthesis (Illumina TotalPrep RNA Amplification Kit - Ambion) following the manufacturer's instructions (IVT: 14h)
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description replicate 1
Data processing The bead intensities were mapped to gene information using BeadStudio 3.2 (Illumina), background correction was perform using the Affymetrix Robust Multi-array Analysis (RMA) background correction model, variance stabilization was performed using the log2 scaling and gene expression normalization was calculated with the quantile method implemented in the lumi package of R-Bioconductor.
Submission date May 17, 2013
Last update date May 13, 2014
Contact name Marcos J Ara├║zo-Bravo
Organization name Max Planck Institute for Molecular Biomedicine
Department Cell and Developmental Biology
Lab Computational Biology and Bioinformatics
Street address Rontgenstr. 20
City Muenster
ZIP/Postal code 48149
Country Germany
Platform ID GPL6885
Series (1)
GSE47061 Dissecting the specific role of Oct4 during the early stage of reprogramming

Data table header descriptions
VALUE quantile normalized

Data table
ILMN_1212607 2.873349
ILMN_1212612 1.744648
ILMN_1212619 3.190733
ILMN_1212628 4.003849
ILMN_1212632 5.84336
ILMN_1212636 12.254018
ILMN_1212637 9.043851
ILMN_1212645 5.375331
ILMN_1212648 7.95545
ILMN_1212653 2.966648
ILMN_1212672 7.8159
ILMN_1212682 3.508603
ILMN_1212683 2.623911
ILMN_1212685 2.206346
ILMN_1212692 7.194727
ILMN_1212693 3.26333
ILMN_1212695 2.627419
ILMN_1212698 2.354427
ILMN_1212702 2.709412
ILMN_1212703 4.517011

Total number of rows: 25697

Table truncated, full table size 551 Kbytes.

Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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