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Sample GSM1143941 Query DataSets for GSM1143941
Status Public on May 13, 2014
Title ESC rep 1
Sample type RNA
 
Source name ESC
Organism Mus musculus
Characteristics cell type: mouse embryonic stem cell
treated: No
Extracted molecule total RNA
Extraction protocol 500 ng of total DNA-free RNA was used as input for labelled cRNA synthesis (Illumina TotalPrep RNA Amplification Kit - Ambion) following the manufacturer's instructions (IVT: 14h)
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description replicate 1
Data processing The bead intensities were mapped to gene information using BeadStudio 3.2 (Illumina), background correction was perform using the Affymetrix Robust Multi-array Analysis (RMA) background correction model, variance stabilization was performed using the log2 scaling and gene expression normalization was calculated with the quantile method implemented in the lumi package of R-Bioconductor.
 
Submission date May 17, 2013
Last update date May 13, 2014
Contact name Marcos J Ara├║zo-Bravo
E-mail(s) mararabra@yahoo.co.uk
Organization name Max Planck Institute for Molecular Biomedicine
Department Cell and Developmental Biology
Lab Computational Biology and Bioinformatics
Street address Rontgenstr. 20
City Muenster
ZIP/Postal code 48149
Country Germany
 
Platform ID GPL6885
Series (1)
GSE47061 Dissecting the specific role of Oct4 during the early stage of reprogramming

Data table header descriptions
ID_REF
VALUE quantile normalized

Data table
ID_REF VALUE
ILMN_1212607 2.873349
ILMN_1212612 1.744648
ILMN_1212619 3.190733
ILMN_1212628 4.003849
ILMN_1212632 5.84336
ILMN_1212636 12.254018
ILMN_1212637 9.043851
ILMN_1212645 5.375331
ILMN_1212648 7.95545
ILMN_1212653 2.966648
ILMN_1212672 7.8159
ILMN_1212682 3.508603
ILMN_1212683 2.623911
ILMN_1212685 2.206346
ILMN_1212692 7.194727
ILMN_1212693 3.26333
ILMN_1212695 2.627419
ILMN_1212698 2.354427
ILMN_1212702 2.709412
ILMN_1212703 4.517011

Total number of rows: 25697

Table truncated, full table size 551 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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