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Sample GSM1143942 Query DataSets for GSM1143942
Status Public on May 13, 2014
Title TO_MEF_0
Sample type RNA
 
Source name TO_MEF_0
Organism Mus musculus
Characteristics cell type: mouse embryonic fibroblast
time: 0
treated: Yes
Extracted molecule total RNA
Extraction protocol 500 ng of total DNA-free RNA was used as input for labelled cRNA synthesis (Illumina TotalPrep RNA Amplification Kit - Ambion) following the manufacturer's instructions (IVT: 14h)
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description replicate 1
Data processing The bead intensities were mapped to gene information using BeadStudio 3.2 (Illumina), background correction was perform using the Affymetrix Robust Multi-array Analysis (RMA) background correction model, variance stabilization was performed using the log2 scaling and gene expression normalization was calculated with the quantile method implemented in the lumi package of R-Bioconductor.
 
Submission date May 17, 2013
Last update date May 13, 2014
Contact name Marcos J Ara├║zo-Bravo
E-mail(s) mararabra@yahoo.co.uk
Organization name Max Planck Institute for Molecular Biomedicine
Department Cell and Developmental Biology
Lab Computational Biology and Bioinformatics
Street address Rontgenstr. 20
City Muenster
ZIP/Postal code 48149
Country Germany
 
Platform ID GPL6885
Series (1)
GSE47061 Dissecting the specific role of Oct4 during the early stage of reprogramming

Data table header descriptions
ID_REF
VALUE quantile normalized

Data table
ID_REF VALUE
ILMN_1212607 2.855521
ILMN_1212612 6.263739
ILMN_1212619 6.044131
ILMN_1212628 3.18149
ILMN_1212632 4.449576
ILMN_1212636 11.742297
ILMN_1212637 9.786254
ILMN_1212645 2.035187
ILMN_1212648 7.325009
ILMN_1212653 7.514868
ILMN_1212672 7.06087
ILMN_1212682 4.375232
ILMN_1212683 1.905725
ILMN_1212685 2.805439
ILMN_1212692 5.44553
ILMN_1212693 7.813184
ILMN_1212695 3.013637
ILMN_1212698 2.474008
ILMN_1212702 2.419249
ILMN_1212703 3.586479

Total number of rows: 25697

Table truncated, full table size 551 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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