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Sample GSM1143944 Query DataSets for GSM1143944
Status Public on May 13, 2014
Title TO_MEF_12
Sample type RNA
Source name TO_MEF_12
Organism Mus musculus
Characteristics cell type: mouse embryonic fibroblast
time: 12
treated: Yes
Extracted molecule total RNA
Extraction protocol 500 ng of total DNA-free RNA was used as input for labelled cRNA synthesis (Illumina TotalPrep RNA Amplification Kit - Ambion) following the manufacturer's instructions (IVT: 14h)
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description replicate 1
Data processing The bead intensities were mapped to gene information using BeadStudio 3.2 (Illumina), background correction was perform using the Affymetrix Robust Multi-array Analysis (RMA) background correction model, variance stabilization was performed using the log2 scaling and gene expression normalization was calculated with the quantile method implemented in the lumi package of R-Bioconductor.
Submission date May 17, 2013
Last update date May 13, 2014
Contact name Marcos J Ara├║zo-Bravo
Organization name Max Planck Institute for Molecular Biomedicine
Department Cell and Developmental Biology
Lab Computational Biology and Bioinformatics
Street address Rontgenstr. 20
City Muenster
ZIP/Postal code 48149
Country Germany
Platform ID GPL6885
Series (1)
GSE47061 Dissecting the specific role of Oct4 during the early stage of reprogramming

Data table header descriptions
VALUE quantile normalized

Data table
ILMN_1212607 2.841165
ILMN_1212612 6.402542
ILMN_1212619 6.566045
ILMN_1212628 3.608182
ILMN_1212632 5.319416
ILMN_1212636 11.654624
ILMN_1212637 9.859101
ILMN_1212645 2.116954
ILMN_1212648 7.832629
ILMN_1212653 7.750551
ILMN_1212672 7.470273
ILMN_1212682 3.075942
ILMN_1212683 2.381838
ILMN_1212685 1.798008
ILMN_1212692 5.465877
ILMN_1212693 6.746955
ILMN_1212695 2.493725
ILMN_1212698 2.178758
ILMN_1212702 2.166757
ILMN_1212703 5.168065

Total number of rows: 25697

Table truncated, full table size 551 Kbytes.

Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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