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Sample GSM1143944 Query DataSets for GSM1143944
Status Public on May 13, 2014
Title TO_MEF_12
Sample type RNA
 
Source name TO_MEF_12
Organism Mus musculus
Characteristics cell type: mouse embryonic fibroblast
time: 12
treated: Yes
Extracted molecule total RNA
Extraction protocol 500 ng of total DNA-free RNA was used as input for labelled cRNA synthesis (Illumina TotalPrep RNA Amplification Kit - Ambion) following the manufacturer's instructions (IVT: 14h)
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description replicate 1
Data processing The bead intensities were mapped to gene information using BeadStudio 3.2 (Illumina), background correction was perform using the Affymetrix Robust Multi-array Analysis (RMA) background correction model, variance stabilization was performed using the log2 scaling and gene expression normalization was calculated with the quantile method implemented in the lumi package of R-Bioconductor.
 
Submission date May 17, 2013
Last update date May 13, 2014
Contact name Marcos J Ara├║zo-Bravo
E-mail(s) mararabra@yahoo.co.uk
Organization name Max Planck Institute for Molecular Biomedicine
Department Cell and Developmental Biology
Lab Computational Biology and Bioinformatics
Street address Rontgenstr. 20
City Muenster
ZIP/Postal code 48149
Country Germany
 
Platform ID GPL6885
Series (1)
GSE47061 Dissecting the specific role of Oct4 during the early stage of reprogramming

Data table header descriptions
ID_REF
VALUE quantile normalized

Data table
ID_REF VALUE
ILMN_1212607 2.841165
ILMN_1212612 6.402542
ILMN_1212619 6.566045
ILMN_1212628 3.608182
ILMN_1212632 5.319416
ILMN_1212636 11.654624
ILMN_1212637 9.859101
ILMN_1212645 2.116954
ILMN_1212648 7.832629
ILMN_1212653 7.750551
ILMN_1212672 7.470273
ILMN_1212682 3.075942
ILMN_1212683 2.381838
ILMN_1212685 1.798008
ILMN_1212692 5.465877
ILMN_1212693 6.746955
ILMN_1212695 2.493725
ILMN_1212698 2.178758
ILMN_1212702 2.166757
ILMN_1212703 5.168065

Total number of rows: 25697

Table truncated, full table size 551 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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