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Sample GSM1143950 Query DataSets for GSM1143950
Status Public on May 13, 2014
Title TO_NSC_0
Sample type RNA
 
Source name TO_NSC_0
Organism Mus musculus
Characteristics cell type: neural stem cell
time: 0
treated: Yes
Extracted molecule total RNA
Extraction protocol 500 ng of total DNA-free RNA was used as input for labelled cRNA synthesis (Illumina TotalPrep RNA Amplification Kit - Ambion) following the manufacturer's instructions (IVT: 14h)
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description replicate 1
Data processing The bead intensities were mapped to gene information using BeadStudio 3.2 (Illumina), background correction was perform using the Affymetrix Robust Multi-array Analysis (RMA) background correction model, variance stabilization was performed using the log2 scaling and gene expression normalization was calculated with the quantile method implemented in the lumi package of R-Bioconductor.
 
Submission date May 17, 2013
Last update date May 13, 2014
Contact name Marcos J Ara├║zo-Bravo
E-mail(s) mararabra@yahoo.co.uk
Organization name Max Planck Institute for Molecular Biomedicine
Department Cell and Developmental Biology
Lab Computational Biology and Bioinformatics
Street address Rontgenstr. 20
City Muenster
ZIP/Postal code 48149
Country Germany
 
Platform ID GPL6885
Series (1)
GSE47061 Dissecting the specific role of Oct4 during the early stage of reprogramming

Data table header descriptions
ID_REF
VALUE quantile normalized

Data table
ID_REF VALUE
ILMN_1212607 3.14565
ILMN_1212612 2.641112
ILMN_1212619 6.490602
ILMN_1212628 2.42033
ILMN_1212632 4.691368
ILMN_1212636 11.690348
ILMN_1212637 9.335591
ILMN_1212645 1.960165
ILMN_1212648 7.691331
ILMN_1212653 9.365086
ILMN_1212672 7.675845
ILMN_1212682 3.327677
ILMN_1212683 2.111748
ILMN_1212685 2.500401
ILMN_1212692 2.820751
ILMN_1212693 7.695522
ILMN_1212695 2.442424
ILMN_1212698 2.486721
ILMN_1212702 2.582321
ILMN_1212703 6.180918

Total number of rows: 25697

Table truncated, full table size 551 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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