NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM1143952 Query DataSets for GSM1143952
Status Public on May 13, 2014
Title TO_NSC_12
Sample type RNA
 
Source name TO_NSC_12
Organism Mus musculus
Characteristics cell type: neural stem cell
time: 12
treated: Yes
Extracted molecule total RNA
Extraction protocol 500 ng of total DNA-free RNA was used as input for labelled cRNA synthesis (Illumina TotalPrep RNA Amplification Kit - Ambion) following the manufacturer's instructions (IVT: 14h)
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description replicate 1
Data processing The bead intensities were mapped to gene information using BeadStudio 3.2 (Illumina), background correction was perform using the Affymetrix Robust Multi-array Analysis (RMA) background correction model, variance stabilization was performed using the log2 scaling and gene expression normalization was calculated with the quantile method implemented in the lumi package of R-Bioconductor.
 
Submission date May 17, 2013
Last update date May 13, 2014
Contact name Marcos J Ara├║zo-Bravo
E-mail(s) mararabra@yahoo.co.uk
Organization name Max Planck Institute for Molecular Biomedicine
Department Cell and Developmental Biology
Lab Computational Biology and Bioinformatics
Street address Rontgenstr. 20
City Muenster
ZIP/Postal code 48149
Country Germany
 
Platform ID GPL6885
Series (1)
GSE47061 Dissecting the specific role of Oct4 during the early stage of reprogramming

Data table header descriptions
ID_REF
VALUE quantile normalized

Data table
ID_REF VALUE
ILMN_1212607 3.044752
ILMN_1212612 5.440634
ILMN_1212619 5.668536
ILMN_1212628 2.682709
ILMN_1212632 3.956378
ILMN_1212636 11.303774
ILMN_1212637 8.876908
ILMN_1212645 1.769289
ILMN_1212648 7.849648
ILMN_1212653 9.657523
ILMN_1212672 6.841824
ILMN_1212682 2.75648
ILMN_1212683 1.835933
ILMN_1212685 3.813727
ILMN_1212692 2.505636
ILMN_1212693 8.321996
ILMN_1212695 2.698366
ILMN_1212698 2.057755
ILMN_1212702 3.201746
ILMN_1212703 6.313376

Total number of rows: 25697

Table truncated, full table size 551 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap