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Sample GSM1143954 Query DataSets for GSM1143954
Status Public on May 13, 2014
Title Ctrl_MEF_0
Sample type RNA
Source name Ctrl_MEF_0
Organism Mus musculus
Characteristics cell type: mouse embryonic fibroblast
time: 0
treated: No
Extracted molecule total RNA
Extraction protocol 500 ng of total DNA-free RNA was used as input for labelled cRNA synthesis (Illumina TotalPrep RNA Amplification Kit - Ambion) following the manufacturer's instructions (IVT: 14h)
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description replicate 1
Data processing The bead intensities were mapped to gene information using BeadStudio 3.2 (Illumina), background correction was perform using the Affymetrix Robust Multi-array Analysis (RMA) background correction model, variance stabilization was performed using the log2 scaling and gene expression normalization was calculated with the quantile method implemented in the lumi package of R-Bioconductor.
Submission date May 17, 2013
Last update date May 13, 2014
Contact name Marcos J Ara├║zo-Bravo
Organization name Max Planck Institute for Molecular Biomedicine
Department Cell and Developmental Biology
Lab Computational Biology and Bioinformatics
Street address Rontgenstr. 20
City Muenster
ZIP/Postal code 48149
Country Germany
Platform ID GPL6885
Series (1)
GSE47061 Dissecting the specific role of Oct4 during the early stage of reprogramming

Data table header descriptions
VALUE quantile normalized

Data table
ILMN_1212607 2.741951
ILMN_1212612 6.704447
ILMN_1212619 6.071252
ILMN_1212628 2.354322
ILMN_1212632 4.253339
ILMN_1212636 11.406521
ILMN_1212637 10.100413
ILMN_1212645 1.773265
ILMN_1212648 7.499813
ILMN_1212653 7.22777
ILMN_1212672 7.004131
ILMN_1212682 4.190797
ILMN_1212683 1.806949
ILMN_1212685 2.428616
ILMN_1212692 5.831842
ILMN_1212693 8.31304
ILMN_1212695 3.010901
ILMN_1212698 1.590175
ILMN_1212702 2.751036
ILMN_1212703 5.030006

Total number of rows: 25697

Table truncated, full table size 551 Kbytes.

Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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