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Sample GSM1143957 Query DataSets for GSM1143957
Status Public on May 13, 2014
Title Ctrl_NSC_24
Sample type RNA
 
Source name Ctrl_NSC_24
Organism Mus musculus
Characteristics cell type: neural stem cell
time: 24
treated: Yes
Extracted molecule total RNA
Extraction protocol 500 ng of total DNA-free RNA was used as input for labelled cRNA synthesis (Illumina TotalPrep RNA Amplification Kit - Ambion) following the manufacturer's instructions (IVT: 14h)
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description replicate 1
Data processing The bead intensities were mapped to gene information using BeadStudio 3.2 (Illumina), background correction was perform using the Affymetrix Robust Multi-array Analysis (RMA) background correction model, variance stabilization was performed using the log2 scaling and gene expression normalization was calculated with the quantile method implemented in the lumi package of R-Bioconductor.
 
Submission date May 17, 2013
Last update date May 13, 2014
Contact name Marcos J Ara├║zo-Bravo
E-mail(s) mararabra@yahoo.co.uk
Organization name Max Planck Institute for Molecular Biomedicine
Department Cell and Developmental Biology
Lab Computational Biology and Bioinformatics
Street address Rontgenstr. 20
City Muenster
ZIP/Postal code 48149
Country Germany
 
Platform ID GPL6885
Series (1)
GSE47061 Dissecting the specific role of Oct4 during the early stage of reprogramming

Data table header descriptions
ID_REF
VALUE quantile normalized

Data table
ID_REF VALUE
ILMN_1212607 3.11918
ILMN_1212612 6.064984
ILMN_1212619 4.557449
ILMN_1212628 2.425902
ILMN_1212632 5.59453
ILMN_1212636 11.455411
ILMN_1212637 9.137034
ILMN_1212645 3.777458
ILMN_1212648 7.546209
ILMN_1212653 10.18343
ILMN_1212672 7.554402
ILMN_1212682 3.145273
ILMN_1212683 1.6743
ILMN_1212685 1.889553
ILMN_1212692 2.002303
ILMN_1212693 7.612505
ILMN_1212695 2.543427
ILMN_1212698 2.302469
ILMN_1212702 1.860797
ILMN_1212703 6.165632

Total number of rows: 25697

Table truncated, full table size 551 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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