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Sample GSM1144523 Query DataSets for GSM1144523
Status Public on Nov 24, 2013
Title rat bladder, 6w obstruction_3A
Sample type RNA
 
Source name urinary bladder, 6w obstruction
Organism Rattus norvegicus
Characteristics strain: Sprague-Dawley
protocol: obstruction
time: 6w
tissue: urinary bladder
Extracted molecule total RNA
Extraction protocol Total RNA from urianry bladder tissue was prepared using the miRNeasy Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer's recommendations
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip rat Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
Description 9_6 w obstruc 3A
miRNA profiling
Data processing The data were analyzed using expression console software version 1.1.2 using default analysis settings and RMA as normalization method.
 
Submission date May 19, 2013
Last update date Nov 24, 2013
Contact name Mari Ekman
E-mail(s) mari.ekman@med.lu.se
Organization name Lund University
Street address BMC D12
City Lund
State/province Sverige
ZIP/Postal code 221 84
Country Sweden
 
Platform ID GPL16384
Series (1)
GSE47080 Partial urethra obstruction in rat

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
api-bantam_st 0.3968801
api-let-7_st 5.192836
api-miR-1000_st 0.5645409
api-miR-100_st 8.821628
api-miR-10_st 7.572675
api-miR-124_st 0.5733184
api-miR-137_st 0.4566651
api-miR-13a_st 0.3612051
api-miR-14_st 0.5673895
api-miR-184a_st 3.255988
api-miR-184b_st 1.208446
api-miR-190_st 0.7184818
api-miR-1923_st 0.3372854
api-miR-1_st 1.170962
api-miR-210_st 1.185805
api-miR-219_st 0.5832817
api-miR-252a_st 0.7176297
api-miR-252b_st 0.5432388
api-miR-263a_st 0.5275311
api-miR-263b_st 0.4598524

Total number of rows: 25015

Table truncated, full table size 652 Kbytes.




Supplementary file Size Download File type/resource
GSM1144523_9_6_w_obstruc_3A.CEL.gz 789.9 Kb (ftp)(http) CEL
Processed data included within Sample table

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