|
| Status |
Public on Nov 24, 2013 |
| Title |
rat bladder, 6w obstruction_5A |
| Sample type |
RNA |
| |
|
| Source name |
urinary bladder, 6w obstruction
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Sprague-Dawley
protocol: obstruction
time: 6w
tissue: urinary bladder
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from urianry bladder tissue was prepared using the miRNeasy Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer's recommendations
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip rat Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneArray Scanner 3000.
|
| Description |
20_6 w obstruc 5A
miRNA profiling
|
| Data processing |
The data were analyzed using expression console software version 1.1.2 using default analysis settings and RMA as normalization method.
|
| |
|
| Submission date |
May 19, 2013 |
| Last update date |
Nov 24, 2013 |
| Contact name |
Mari Ekman |
| E-mail(s) |
mari.ekman@med.lu.se
|
| Organization name |
Lund University
|
| Street address |
BMC D12
|
| City |
Lund |
| State/province |
Sverige |
| ZIP/Postal code |
221 84 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL16384 |
| Series (1) |
| GSE47080 |
Partial urethra obstruction in rat |
|
