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Sample GSM1144755 Query DataSets for GSM1144755
Status Public on May 21, 2013
Title Pooled sample at d0, left plantaris, young mice
Sample type RNA
 
Source name Left plantaris muscle, d0
Organism Mus musculus
Characteristics strain/background: C57BL/6J
gender: male
tissue: skeletal muscle (left plantaris)
age: 5 months
time point: day 0
Growth protocol Male C57BL/6J mice (The Jackson Laboratory, Bar Harbor, ME), 5 months of age were housed in a temperature- and humidity-controlled facility on a 14:10 h light:dark cycle with access to food and water ad libitum.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cDNA were prepared according to the standard Ambion and Affymetrix protocol from 250 ng total RNA (Ambion WT Expression kit and Affymetrix WT Terminal Labeling kit).
 
Hybridization protocol Following fragmentation, 2.5 ug of cDNA were hybridized for 17 hr at 45C on GeneChip Mouse Gene 1.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip 7G Scanner.
Description Overload d0, left
Gene expression data from left plantaris muscle, d0
Data processing The CEL data were generated using Affymetrix Command Console software and the data analysis was performed using Partek Genomics Suite.
 
Submission date May 20, 2013
Last update date May 21, 2013
Contact name Thomas Chaillou
E-mail(s) thomas.chaillou@uky.edu
Organization name Department of Physiology
Street address University of Kentucky, Department of Physiology, 800 Rose Street
City Lexington
State/province KY
ZIP/Postal code 40536-0298
Country USA
 
Platform ID GPL6246
Series (1)
GSE47098 Expression data during plantaris muscle hypertrophy induced by synergist ablation in young adult mice

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
10338001 7406.6
10338002 313.5
10338003 2656.7
10338004 1309.0
10338005 9.1
10338006 12.1
10338007 22.3
10338008 54.1
10338009 1084.5
10338010 9.3
10338011 229.2
10338012 10.5
10338013 8.2
10338014 8.4
10338015 8.1
10338016 728.4
10338017 11970.5
10338018 445.7
10338019 161.3
10338020 1022.6

Total number of rows: 35556

Table truncated, full table size 518 Kbytes.




Supplementary file Size Download File type/resource
GSM1144755_5-0L.CEL.gz 4.4 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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