|
| Status |
Public on May 21, 2013 |
| Title |
Pooled sample at d3, left plantaris, young mice |
| Sample type |
RNA |
| |
|
| Source name |
Left plantaris muscle, d3
|
| Organism |
Mus musculus |
| Characteristics |
strain/background: C57BL/6J
gender: male
tissue: skeletal muscle (left plantaris)
age: 5 months
treatment: muscle hypertrophy induced by synergist ablation
time point: day 3
|
| Growth protocol |
Male C57BL/6J mice (The Jackson Laboratory, Bar Harbor, ME), 5 months of age were housed in a temperature- and humidity-controlled facility on a 14:10 h light:dark cycle with access to food and water ad libitum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the standard Ambion and Affymetrix protocol from 250 ng total RNA (Ambion WT Expression kit and Affymetrix WT Terminal Labeling kit).
|
| |
|
| Hybridization protocol |
Following fragmentation, 2.5 ug of cDNA were hybridized for 17 hr at 45C on GeneChip Mouse Gene 1.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip 7G Scanner.
|
| Description |
Overload d3, left
Gene expression data from left plantaris muscle, d3
|
| Data processing |
The CEL data were generated using Affymetrix Command Console software and the data analysis was performed using Partek Genomics Suite.
|
| |
|
| Submission date |
May 20, 2013 |
| Last update date |
May 21, 2013 |
| Contact name |
Thomas Chaillou |
| E-mail(s) |
thomas.chaillou@uky.edu
|
| Organization name |
Department of Physiology
|
| Street address |
University of Kentucky, Department of Physiology, 800 Rose Street
|
| City |
Lexington |
| State/province |
KY |
| ZIP/Postal code |
40536-0298 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE47098 |
Expression data during plantaris muscle hypertrophy induced by synergist ablation in young adult mice |
|
