|
| Status |
Public on Aug 20, 2013 |
| Title |
non-endocrine neonatal islet cells from postnatal day 2-3 pancreas of male and female rats, bio rep 3 |
| Sample type |
RNA |
| |
|
| Source name |
non-endocrine neonatal islet cells: cells co-isolated with the endocrine cells, and FACS-sorted for their low side scatter (LSSC), from postnatal day 2-3 pancreas of male and female Wistar rats
|
| Organism |
Rattus norvegicus |
| Characteristics |
cell type: non-endocrine neonatal islet cells
gender: male and female
strain: Wistar
age: 2-3 d
|
| Treatment protocol |
None, freshly isolated beta cells snap frozen in liquid nitrogen immediately after isolation
|
| Growth protocol |
Pancreatic beta cells were FACS-purified from neonatal (postnatal day 2-3)and 10-weeks old Wistar rat pancreas, and immediately snap frozen in liquid nitrogen, prior to total RNA extraction in batch, from all biological replicates.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNEasy extraction of total RNA was performed according to the manufacturer's instructions. RNA integrity verified on Bioanalyzer with minimal RIN cutoff = 8.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
After fragmentation at 94°C for 35 min in fragmentation buffer (40mM Tris, 30mM magnesium acetate, 10mM potassium acetate), the labelled cRNA was hybridized for 16h to the Affymetrix HG133A
|
| Scan protocol |
Arrays were stained with phycoerythrin-streptavidin with the Affymetrix Fluidics Station 400, and scanned in the Affymetrix GeneArray2500scanner.
|
| Description |
non-endocrine neonatal islet cells: cells co-isolated with the endocrine cells, and FACS-sorted for their low side scatter (LSSC), from postnatal day 2-3 pancreas of male and female Wistar rats, biological replicate 3, pool of 30 animals
|
| Data processing |
Scanned arrays were analyzed with dChip model-based expression analysis to the array with the median intensity and correction for mismatch hybridization using the perfect match-mismatch correction option.
|
| |
|
| Submission date |
May 22, 2013 |
| Last update date |
Aug 20, 2013 |
| Contact name |
Geert A. Martens |
| E-mail(s) |
geert.martens@vub.ac.be
|
| Organization name |
Vrije Universiteit Brussel
|
| Department |
Diabetes Research Center
|
| Street address |
Laarbeeklaan 103
|
| City |
Brussels |
| ZIP/Postal code |
1090 |
| Country |
Belgium |
| |
|
| Platform ID |
GPL1355 |
| Series (1) |
| GSE47174 |
Genome-wide mRNA expression profiles of FACS-enriched rat beta cells freshly isolated from neonatal (postnatal day 2-3) and adult (10 weeks-old) Wistar rats |
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