|
| Status |
Public on May 25, 2013 |
| Title |
3D7SET8KO_18h, Lot#1 |
| Sample type |
RNA |
| |
|
| Source name |
PfSET8 knockout P. falciparum, 18h post invation
|
| Organism |
Plasmodium falciparum 3D7 |
| Characteristics |
strain: 3D7
Stage: ring
genotype/variation: PfSET8 knockout
|
| Treatment protocol |
Parasites were synchronized to ring stage by 5% D-sorbitol treatment at 37C for 10 min at least one asexual cycle prior to sample collection.
|
| Growth protocol |
P. falciparum clones were cultured in the host erythrocytes at 37C and collected at different indicated time points post invasion.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix 3'IVT express protocol from 250ng total RNA (GeneChip® 3’ IVT Express Kit User Manual).
|
| |
|
| Hybridization protocol |
15ug fragmented cRNA was hybridized on Affymetrix Plasmodium array following Manufacture's protocol.
|
| Scan protocol |
Array was scanned on Affymetrix Gene ChIP Scanner 3000 7G.
|
| Description |
Gene expression data from P.falciparum parasite clones at the asexual stage.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
May 24, 2013 |
| Last update date |
May 25, 2013 |
| Contact name |
YAN LUO |
| E-mail(s) |
yan.luo@nih.gov
|
| Organization name |
NHLBI
|
| Street address |
9000 Rockville Pike
|
| City |
Bethesda |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL1321 |
| Series (1) |
| GSE47349 |
Expression data of gene knockout Plasmodium falciparum clones |
|
