|
Status |
Public on Jun 21, 2006 |
Title |
Mammary MT1-MMP rep2 |
Sample type |
RNA |
|
|
Source name |
cell line 184B5-MT
|
Organism |
Homo sapiens |
Characteristics |
cell line 184B5-MT. Mammary epithelial. Gender: Female. Age: Derived from 184B5 by transfection with a full length MT1-MMP lentiviral construct.
|
Extracted molecule |
total RNA |
Extraction protocol |
Illumina TotalPrep RNA amplification kit (Ambion Catalog#IL1791)
|
Label |
Streptavidin Cy3
|
Label protocol |
As described in the illumina Human-6 chip processing protocol. Briefly, generation of biotin labelled cRNA from the product of RT cDNA synthesis and 2nd strand cDNA synthesis.
|
|
|
Hybridization protocol |
As described in the illumina Human-6 hybridisation protocol. Doc#11176837 Rev E.
|
Scan protocol |
As suggested by Illumina using the Illumina scanner.
|
Description |
Second biological replicate from three for the transfected 184B5-MT cell line
|
Data processing |
None: raw data from Beadstudio
|
|
|
Submission date |
Jun 16, 2006 |
Last update date |
Jun 20, 2006 |
Contact name |
Roy M Williams |
E-mail(s) |
rwillia@scripps.edu
|
Organization name |
The Scripps Research Institute
|
Department |
Regenerative Medicine
|
Lab |
Loring
|
Street address |
3030 Science Park Road
|
City |
La Jolla |
State/province |
CA |
ZIP/Postal code |
92037 |
Country |
USA |
|
|
Platform ID |
GPL2507 |
Series (1) |
GSE5095 |
MT1-MMP induces malignant transformation in Mammary cells |
|