|
| Status |
Public on Feb 15, 2007 |
| Title |
Endometriosis 5B WG |
| Sample type |
RNA |
| |
|
| Source name |
endometriosis
|
| Organism |
Homo sapiens |
| Characteristics |
Human, female, of reproductive age, severe endometriosis
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The eutopic and ectopic endometrial samples were placed in RNAlater (Ambion) in the surgical suite. The endometrial samples were homogenized in TRI reagent (MRC) using a Polytron (Brinkmann). The RNA-containing layer was removed and the RNA purified on an RNeasy extraction column (Qiagen). The sample was treated with an on-column DNase treatment (RNase-free DNase, Qiagen). The purity and quantity was evaluated with the RNA 6000 NanoLabChip in an Agilent Bioanalyzer.
|
| Label |
Alexa-647
|
| Label protocol |
see CodeLink application notes and manuals
|
| |
|
| Hybridization protocol |
see CodeLink application notes and manuals
|
| Scan protocol |
see CodeLink application notes and manuals
|
| Description |
Each patient served as her own control; the eutopic endometrial biopsies were obtained from the patients at the same surgery in which the endometriotic explants were excised. IRB approval and informed consent were obtained for all sample collection.
|
| Data processing |
global median normalization, CodeLink Expression Analysis 4.1.0.4163
|
| |
|
| Submission date |
Jun 16, 2006 |
| Last update date |
Feb 12, 2009 |
| Contact name |
Kathleen M Eyster |
| E-mail(s) |
Kathleen.Eyster@usd.edu
|
| Organization name |
University of South Dakota
|
| Department |
Basic Biomedical Sciences
|
| Street address |
414 E. Clark St.
|
| City |
Vermillion |
| State/province |
SD |
| ZIP/Postal code |
57069 |
| Country |
USA |
| |
|
| Platform ID |
GPL2895 |
| Series (1) |
| GSE5108 |
Whole human genome DNA microarray analysis of gene expression in ectopic vs. eutopic endometrium |
|
