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Sample GSM115047 Query DataSets for GSM115047
Status Public on Jul 10, 2006
Title Monocyte at T0, biological rep2 HG-U133A
Sample type RNA
 
Source name Blood
Organism Homo sapiens
Characteristics Monocyte at T0, biological rep2
Treatment protocol freshly isolated monocytes
Growth protocol Cells were cultured in Petriperm dishes tomimic circulating conditions
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip HGU133 series. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description Monocytes
Data processing The data were analyzed with RNA using Bioconductor's R.
 
Submission date Jun 19, 2006
Last update date Jul 15, 2019
Contact name Fernando O Martinez Estrada
E-mail(s) f.martinezestrada@surrey.ac.uk
Organization name University of Surrey
Department Faculty of Health & Medical Sciences
Lab Innate Immunity
Street address Stag Hill
City Guildford
State/province Surrey
ZIP/Postal code GU2 7XH
Country United Kingdom
 
Platform ID GPL96
Series (2)
GSE5099 Expression Data from Macrophage Maturation and Polarization Experiment
GSE35495 Genome-wide analysis of human and mouse macrophages, resting and activated
Relations
Reanalyzed by GSE86363
Reanalyzed by GSE134312

Data table header descriptions
ID_REF
VALUE RMA-calculated Signal intensity

Data table
ID_REF VALUE
1007_s_at 7.883269075
1053_at 7.640917363
117_at 9.417377256
121_at 9.164025463
1255_g_at 4.757915831
1294_at 8.354779636
1316_at 6.876587044
1320_at 5.537538495
1405_i_at 5.845319557
1431_at 4.321411324
1438_at 7.642387995
1487_at 9.235458877
1494_f_at 7.049640033
1598_g_at 9.436190204
160020_at 9.541000075
1729_at 9.437962619
177_at 5.619754131
1773_at 6.98954704
179_at 9.68251478
1861_at 6.811428219

Total number of rows: 22283

Table truncated, full table size 496 Kbytes.




Supplementary file Size Download File type/resource
GSM115047_M0_D2_chipA.CEL.gz 3.5 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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