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Sample GSM115049 Query DataSets for GSM115049
Status Public on Jul 10, 2006
Title Monocyte at 3 days, biological rep1 HG-U133A
Sample type RNA
 
Source name Blood
Organism Homo sapiens
Characteristics Monocyte at 3 days, biological rep1
Treatment protocol monocytes stimulated for 3 days with 100 ng/ml M-CSF
Growth protocol RPMI 1640 20%FCS 100 ng/ml M-CSF
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip HGU133 series. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description Monocytes
Data processing The data were analyzed with RNA using Bioconductor's R.
 
Submission date Jun 19, 2006
Last update date Jul 15, 2019
Contact name Fernando O Martinez Estrada
E-mail(s) f.martinezestrada@surrey.ac.uk
Organization name University of Surrey
Department Faculty of Health & Medical Sciences
Lab Innate Immunity
Street address Stag Hill
City Guildford
State/province Surrey
ZIP/Postal code GU2 7XH
Country United Kingdom
 
Platform ID GPL96
Series (2)
GSE5099 Expression Data from Macrophage Maturation and Polarization Experiment
GSE35495 Genome-wide analysis of human and mouse macrophages, resting and activated
Relations
Reanalyzed by GSE134312

Data table header descriptions
ID_REF
VALUE RMA-calculated Signal intensity

Data table
ID_REF VALUE
1007_s_at 7.92913121
1053_at 8.21669469
117_at 8.330896218
121_at 9.581511569
1255_g_at 4.769197728
1294_at 8.898810973
1316_at 6.845319954
1320_at 5.349505472
1405_i_at 5.581760327
1431_at 4.336814475
1438_at 7.365245365
1487_at 8.851938178
1494_f_at 7.175216316
1598_g_at 10.7157515
160020_at 9.812316291
1729_at 9.128212356
177_at 7.069522221
1773_at 7.502408208
179_at 9.710119548
1861_at 7.172464442

Total number of rows: 22283

Table truncated, full table size 496 Kbytes.




Supplementary file Size Download File type/resource
GSM115049_Md3_D1_chipA.CEL.gz 3.6 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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