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Sample GSM115050 Query DataSets for GSM115050
Status Public on Jul 10, 2006
Title Monocyteat 3 days, biological rep2 HG-U133A
Sample type RNA
 
Source name Blood
Organism Homo sapiens
Characteristics Monocyteat 3 days, biological rep2
Treatment protocol monocytes stimulated for 3 days with 100 ng/ml M-CSF
Growth protocol RPMI 1640 20%FCS 100 ng/ml M-CSF
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip HGU133 series. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description Monocytes
Data processing The data were analyzed with RNA using Bioconductor's R.
 
Submission date Jun 19, 2006
Last update date Jul 15, 2019
Contact name Fernando O Martinez Estrada
E-mail(s) f.martinezestrada@surrey.ac.uk
Organization name University of Surrey
Department Faculty of Health & Medical Sciences
Lab Innate Immunity
Street address Stag Hill
City Guildford
State/province Surrey
ZIP/Postal code GU2 7XH
Country United Kingdom
 
Platform ID GPL96
Series (2)
GSE5099 Expression Data from Macrophage Maturation and Polarization Experiment
GSE35495 Genome-wide analysis of human and mouse macrophages, resting and activated
Relations
Reanalyzed by GSE86363
Reanalyzed by GSE134312

Data table header descriptions
ID_REF
VALUE RMA-calculated Signal intensity

Data table
ID_REF VALUE
1007_s_at 7.786254025
1053_at 8.405602886
117_at 8.991076605
121_at 9.877934895
1255_g_at 4.742793921
1294_at 9.044877839
1316_at 6.708851886
1320_at 5.471020175
1405_i_at 5.519416394
1431_at 4.340026637
1438_at 7.253738846
1487_at 9.078229064
1494_f_at 7.20933711
1598_g_at 10.28177849
160020_at 9.917397841
1729_at 9.443239281
177_at 7.088854067
1773_at 7.553983061
179_at 9.487187736
1861_at 6.998601137

Total number of rows: 22283

Table truncated, full table size 496 Kbytes.




Supplementary file Size Download File type/resource
GSM115050_Md3_D2_chipA.CEL.gz 3.5 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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